Abstract
Lymphocytes separated from fresh heparinized blood or from blood stored 24 h at room temperature were tested for E rosettes, EAC Rosettes, and blastogenic response to phytohemagglutinin. Compared to lymphocytes from fresh blood, lymphocytes from 24 h stored blood contained a lower percentage of E rosette-positive cells, a higher percentage of E rosette-negative EAC rosette-negative null cells, and manifested a decreased blastogenic response to phytohemagglutinin. Fewer viable mononuclear cells were recovered by Ficoll-Hypaque separation from stored blood than from fresh blood. These findings suggest that storage-related changes may occur in T cells which cause a selective loss of such cells during Ficoll-Hypaque separation. A delay in processing of blood samples may therefore yield results of lymphocyte marker and functional assays which give the false impression of a decrease in T cells and an increase in null cells.
Citations
Dec 20, 1999·Analytical Biochemistry·H RiolE Wang
Apr 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A KleinA Malkin
Oct 24, 1985·Journal of Immunological Methods·A J GearingR Thorpe
Oct 3, 1999·Veterinary Immunology and Immunopathology·D DalleyR G Hewinson
Jan 1, 1992·Australian Veterinary Journal·J S RothelP R Wood
Jul 1, 1985·The Journal of Clinical Investigation·B L DavidsonG Rovera
Mar 10, 2011·Journal of Translational Medicine·Walter C OlsonCraig L Slingluff
Dec 2, 2008·Journal of Immunological Methods·Kyle C McKennaRichard A Bilonick
Jun 1, 1997·The Journal of Laboratory and Clinical Medicine·G J BrewerJ Kaplan
Apr 1, 1987·Clinical Immunology and Immunopathology·S M Lipson
Mar 15, 2015·Blood·Gertjan LugthartMarco W Schilham
Apr 7, 2009·Current Protocols in Immunology·Ivan J FussHeddy Zola
Apr 25, 2008·Current Protocols in Immunology·M E KanofH Zola
Jul 7, 2000·Clinical and Diagnostic Laboratory Immunology·P Kumar, V Satchidanandam
Aug 13, 2020·Biology·Nour Z AtwanyMona Rushdi Hassuneh
Jan 20, 2019·BMC Immunology·Adriana NavasMaría Adelaida Gómez
Sep 21, 2017·Journal of Immunological Methods·R Madelaine ParedesForest Sheppard
Apr 6, 2021·Journal of Immunological Methods·Troy D QuerecElizabeth R Unger