PMID: 2501783Jul 1, 1989Paper

Altered membrane association of p60v-src and a murine 63-kDa N-myristoyl protein after incorporation of an oxygen-substituted analog of myristic acid

Proceedings of the National Academy of Sciences of the United States of America
R O Heuckeroth, J I Gordon

Abstract

A number of viral and cellular proteins contain covalently bound lipid. In a subset of these acyl proteins, the 14-carbon saturated fatty acid myristic acid is attached through an amide linkage to an NH2-terminal glycine residue. Myristoyl-CoA:protein N-myristoyltransferase (NMT) transfers the myristoyl moiety from myristoyl-CoA to these nascent proteins and is highly selective for fatty acid chain length. We have found that 10-(propoxy)decanoyl-CoA (11-oxymyristoyl-CoA), an analog of myristic acid with reduced hydrophobicity, acts as a substrate for NMT both in vitro and in vivo. Comparison of the in vitro kinetic properties of a number of synthetic octapeptide substrates of NMT using myristoyl-CoA or 11-oxymyristoyl-CoA indicated that there is an interaction between the acyl-CoA and peptide binding sites of this acyltransferase. Peptide catalytic efficiency with 11-oxymyristoyl-CoA was reduced relative to that with myristoyl-CoA, but the extent of the reduction varied widely among the octapeptides tested. These in vitro data accurately predicted that only a subset of myristoyl proteins synthesized in Saccharomyces cerevisiae and a murine myocyte-like cell line (BC3H1) would incorporate 11-oxy[3H]myristate. Substitution of the...Continue Reading

References

Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D PellmanH Hanafusa
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M P KampsB M Sefton
Jan 1, 1988·Annual Review of Biochemistry·D A TowlerL Glaser
Jan 1, 1988·Annual Review of Cell Biology·A M SchultzS Oroszlan
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A TowlerJ I Gordon
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R O HeuckerothJ I Gordon
Jun 1, 1987·The Journal of Cell Biology·B M Sefton, J E Buss
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A ReinA M Schultz
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Towler, L Glaser
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B A HemmingsE W Jones

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Citations

Jan 1, 1997·Cellular Signalling·J A Boutin
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BryantJ I Gordon
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J DuronioJ I Gordon
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·T L DoeringP T Englund
Oct 1, 1990·Trends in Biochemical Sciences·R A McIlhinney
Dec 20, 2007·The Journal of General Virology·Kelly E Seaton, Charles D Smith
Dec 1, 1990·Current Opinion in Cell Biology·R J DeschenesJ R Broach
Jan 16, 2004·The Journal of Pharmacology and Experimental Therapeutics·Kevin J FrenchCharles D Smith
Jun 30, 1995·The Journal of Biological Chemistry·K SanadaY Fukada

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