PMID: 8316231Apr 21, 1993

Altered membrane fluidity in rat hepatocytes during endotoxic shock

Molecular and Cellular Biochemistry
R SalgiaM M Sayeed

Abstract

Steady-state fluorescence anisotropy measurements of the fluorescent hydrocarbon probe 1,6-diphenyl-1,3,4-hexatriene (DPH) were carried out in isolated hepatocytes of saline control and Salmonella enteritidis endotoxin (20 mg/kg) injected rats. Statistically significant differences were observed in the fluorescent anisotropy (rs) and membrane microviscosity (eta) values of control (rs = 0.107 +/- 0.004 (SEM), eta = 0.98 +/- 0.08, n +/- 6) versus endotoxin injected rat hepatocytes (rs = 0.134 +/- 0.005, eta = 1.43 +/- 0.08, n = 6, p < 0.001) at 37 degrees C. Fluidity was similarly lower in the isolated plasma membrane preparations from endotoxin-injected rat livers relative to control livers. When endotoxin-injected rats were treated with the calcium channel-blocker diltiazem, the anisotropy and microviscosity values were comparable to those obtained from control rats (rs = 0.152 +/- 0.003, eta = 1.00 +/- 0.003, n = 6). These measurements were made in animals five hours after endotoxin had been injected, and thus represent the in vivo effects of bacterial endotoxins. Temperature scan studies of DPH from 5-40 degrees C revealed that the membrane fluidity of endotoxin-injected rat hepatocytes was significantly lower than control h...Continue Reading

References

Dec 15, 1978·Biochimica Et Biophysica Acta·M Shinitzky, Y Barenholz
Mar 20, 1974·Biochimica Et Biophysica Acta·S J PilkisC R Park
Jan 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D Schachter
Jul 28, 1983·The American Journal of Medicine·W R McCabeA De Maria
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Dec 1, 1970·The Journal of Membrane Biology·B G SchusterR S Aronson

Citations

Aug 16, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mathieu VinkenVera Rogiers
Feb 5, 2008·Clinics in Liver Disease·Michael Fuchs, Arun J Sanyal
Nov 14, 1997·The Journal of Surgical Research·M SilomonS Rose
Jul 9, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rajkumar CheluvappaDavid G Le Couteur
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E SturmJ M Crawford
Dec 26, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nisha Chand, Arun J Sanyal

Related Concepts

Metazoa
Aldizem
Fluorescence Polarization
Liver
Membrane Fluidity
August Rats
Endotoxic Shock
Rats, Laboratory

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