PMID: 7537859May 1, 1995Paper

Altered metaphase chromosome structure in xrs-5 cells is not related to its radiation sensitivity or defective DNA break rejoining

Mutation Research
J L SchwartzB A Sedita

Abstract

The chinese hamster ovary (CHO) cell line xrs-5 is a radiation-sensitive derivative of CHO-K1 cells. The xrs-5 cells have a defect in DNA double-strand break rejoining and show alterations in chromosome structure and nuclear morphology. The relationship between radiation sensitivity and metaphase chromosome morphology was examined in 12 'revertant' xrs-5 clones isolated following treatment with 5-azacytidine. nine of the clones were radioresistant while the other three retained xrs-5-like radiation sensitivity. Chromosome morphology reverted to CHO-K1-like characteristics in three of the radioresistant clones and one of the radiosensitive clones suggesting that the over-condensed metaphase chromosome morphology of xrs-5 cells does not underlie its radiation sensitivity. Radiation sensitivity did correlate with DNA double-strand break rejoining ability. The radioresistant clones showing the over-condensed xrs-5-like chromosome morphology were also slightly more sensitive to the topoisomerase II inhibitor etoposide (VP-16) than CHO-K1, suggesting that the over-condensed morphology might be due to alterations in the phosphorylation of chromatin proteins.

References

May 25, 1989·Nature·A W Murray, M W Kirschner
Nov 1, 1994·Mutation Research·J L SchwartzD J Grdina
Jan 1, 1993·Environmental and Molecular Mutagenesis·J L Schwartz, A T Vaughan

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Citations

Mar 5, 2003·Teratogenesis, Carcinogenesis, and Mutagenesis·A P A GuimarãesE T Sakamoto-Hojo
Oct 3, 2012·PloS One·Yongfeng LiFrancis A Cucinotta

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