Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity

Journal of Diabetes Research
Mette J JacobsenDavid Simar

Abstract

Obesity is associated with immunological perturbations that contribute to insulin resistance. Epigenetic mechanisms can control immune functions and have been linked to metabolic complications, although their contribution to insulin resistance still remains unclear. In this study, we investigated the link between metabolic dysfunction and immune alterations with the epigenetic signature in leukocytes in a porcine model of obesity. Global DNA methylation of circulating leukocytes, adipose tissue leukocyte trafficking, and macrophage polarisation were established by flow cytometry. Adipose tissue inflammation and metabolic function were further characterised by quantification of metabolites and expression levels of genes associated with obesity and inflammation. Here we show that obese pigs showed bigger visceral fat pads, higher levels of circulating LDL cholesterol, and impaired glucose tolerance. These changes coincided with impaired metabolism, sustained macrophages infiltration, and increased inflammation in the adipose tissue. Those immune alterations were linked to global DNA hypermethylation in both B-cells and T-cells. Our results provide novel insight into the possible contribution of immune cell epigenetics into the im...Continue Reading

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Citations

Nov 14, 2016·Environmental Research·Laura CantoneValentina Bollati
Oct 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Audrey PoupeauRomain Barrès
Aug 8, 2019·The Journal of Clinical Endocrinology and Metabolism·Danielle HiamNigel K Stepto
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Nov 25, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Omar Ramos-LopezJ Alfredo Martinez
Oct 23, 2020·Nutrition & Metabolism·Yang XiaoElizabeth R Gilbert

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Methods Mentioned

BETA
Flow Cytometry
chip
FACS

Software Mentioned

FlowJo
SPSS Statistics
Genex5 Pro

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