Altered Micro-RNA Regulation and Neuroprotection Activity of Eremostachys labiosiformis in Alzheimer's Disease Model

Annals of Neurosciences
Mohammad Rasoul Samandari-BahrasemanLeila Elyasi

Abstract

Amyloid-β peptide (Aβ) is involved in the formation of senile plaques in Alzheimer's disease (AD), and causes neuronal cell death by inducing oxidative stress. We investigated the protective effect of Eremostachys labiosiformis extract against the Aβ-induced toxicity in SH-SY5Y cells. Methanolic extract from the aerial parts of E. labiosiformis was prepared by percolation method at room temperature. SH-SY5Y cells were treated and incubated with different concentrations of the extract for 1 h, before addition of Aβ. Cytotoxicity was measured 24 h after the addition of Aβ to the medium using MTT and reactive oxygen species (ROS) assays. Effective doses were evaluated using real-time polymerase chain reaction to evaluate expression of miR-212 and miR-132. The results were analyzed using SPSS software (16). Exposure of -SH-SY5Y cells to Aβ significantly affected the viability of cells and increased ROS levels. The results revealed that 1.2 and 2.5 μg/mL of the E. labiosiformis extract reduced Aβ-induced deterioration. Only 2.5 μg/mL of the extract could reduce ROS levels. In addition, 5 μg/mL of the extract increased the expression of the miRNAs, which was reduced after exposure to Aβ. Based on the antioxidant and protective effect...Continue Reading

References

Mar 20, 2001·Archives of Neurology·J C MorrisL Berg
Jun 14, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Maria E Bamberger, Gary E Landreth
Oct 23, 2003·Nature Reviews. Cancer·Young-Joon Surh
Nov 29, 2007·Expert Opinion on Investigational Drugs·Cesare MancusoVittorio Calabrese
Oct 17, 2009·Neurobiology of Disease·Brent Cameron, Gary E Landreth
Nov 3, 2009·Current Genomics·Olivier C MaesHyman M Schipper
Jan 7, 2010·Journal of Neurochemistry·Jiyoung KimKi Won Lee
Oct 29, 2010·Journal of Physiology and Biochemistry·Yong HuangGuo Zheng Zhang
Jun 7, 2011·Drugs & Aging·Melanie-Jayne R Howes, Elaine Perry
Jan 31, 2012·Neurobiology of Disease·Charlotte DelaySébastien S Hébert
Mar 1, 2012·Nucleic Acids Research·Anaïs WanetPatricia Renard
Mar 20, 2012·Cell·Yadong Huang, Lennart Mucke
Apr 16, 2013·Human Molecular Genetics·Hon-Kit Andus WongAnna M Krichevsky
Sep 13, 2015·Human Molecular Genetics·Pascal Y SmithSébastien S Hébert

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