Altered monocyte expression and expansion of non-classical monocyte subset in IgA nephropathy patients

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Sharon N CoxF P Schena

Abstract

The main defect of immunoglobulin A nephropathy (IgAN) lies within the immune system and in peripheral blood mononuclear cells rather than in the kidney. Previously, we found an altered gene expression in monocytes compared with B and T cells isolated from IgAN patients; thus, our aim here has been to study this subset at a genome-wide and functional level. A total of 39 IgAN patients and 37 healthy blood donors (HBDs) were included in this study, and microarray technology was used to evaluate global gene expression differences in monocytes isolated from IgAN patients and HBDs. Aberrantly expressed genes and pathways were then validated on an independent set of IgAN patients with RT-PCR western blot and flow cytometric analysis. Gene expression differences in monocytes from IgAN patients and HBDs primarily involved apoptosis signalling, mitochondrial dysfunction, tnfr2/1 and death receptor signalling. Both the extrinsic and intrinsic apoptotic pathways seem to be implicated; in particular, the protein levels of NDUFS3 and TNFRSF1A were upregulated thus confirming the altered mitochondrial and death receptor homeostasis. Furthermore, the basal intracellular protein levels of TNF in monocytes were lower in IgAN patients compared ...Continue Reading

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Citations

Oct 13, 2016·Journal of Internal Medicine·S N CoxUNKNOWN European IgAN Consortium
Feb 6, 2016·Clinical Science·Fabio SallustioFrancesco P Schena
Aug 24, 2017·Scientific Reports·Magdalena KrochmalJoachim Jankowski
Feb 23, 2020·International Journal of Molecular Sciences·Clara Esteve ColsEva Mª Martínez Cáceres
Jun 16, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Francesco Paolo SchenaSharon N Cox
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Laura J SmythAmy Jayne McKnight
Feb 9, 2021·Frontiers in Endocrinology·Bin HuRuifeng Shi
Oct 31, 2020·Frontiers in Immunology·Haresh SelvaskandanJonathan Barratt

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