PMID: 11336920May 5, 2001Paper

Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading

Bone
H B Sun, H Yokota

Abstract

In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.

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Citations

Sep 10, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel J LeongHui B Sun
Oct 17, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Dennis R Carter, Marcy Wong
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Yuan-Ping Han
Jul 28, 2011·Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders·Hui B SunHiroki Yokota
Oct 30, 2004·Connective Tissue Research·Masaru YamaguchiKazutaka Kasai
Apr 25, 2007·Journal of Cellular Biochemistry·Kanokwan Charoonpatrapong-PanyayongJoseph P Bidwell
Apr 16, 2010·Annals of the New York Academy of Sciences·Hui B Sun
Apr 1, 2006·Biochemical and Biophysical Research Communications·Masaji MoritaKatsuji Shimizu
Mar 21, 2019·Annals of the New York Academy of Sciences·Lidi LiuHui B Sun
Mar 21, 2019·Annals of the New York Academy of Sciences·Zhiyong HeHui B Sun
Nov 10, 2004·Clinical Orthopaedics and Related Research·Diah S BramonoGregory H Altman
Aug 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ting LiuYong-Jun Li

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