Altered neurotransmitter release, vesicle recycling and presynaptic structure in the pilocarpine model of temporal lobe epilepsy.

Brain : a Journal of Neurology
Chirag UpretiEmilio R Garrido-Sanabria

Abstract

In searching for persistent seizure-induced alterations in brain function that might be causally related to epilepsy, presynaptic transmitter release has relatively been neglected. To measure directly the long-term effects of pilocarpine-induced status epilepticus on vesicular release and recycling in hippocampal mossy fibre presynaptic boutons, we used (i) two-photon imaging of FM1-43 vesicular release in rat hippocampal slices; and (ii) transgenic mice expressing the genetically encoded pH-sensitive fluorescent reporter synaptopHluorin preferentially at glutamatergic synapses. In this study we found that, 1-2 months after pilocarpine-induced status epilepticus, there were significant increases in mossy fibre bouton size, faster rates of action potential-driven vesicular release and endocytosis. We also analysed the ultrastructure of rat mossy fibre boutons using transmission electron microscopy. Pilocarpine-induced status epilepticus led to a significant increase in the number of release sites, active zone length, postsynaptic density area and number of vesicles in the readily releasable and recycling pools, all correlated with increased release probability. Our data show that presynaptic release machinery is persistently alt...Continue Reading

References

Jan 1, 1990·Neuroscience and Biobehavioral Reviews·J P LeiteE A Cavalheiro
Dec 15, 1989·Neuroscience Letters·J HovorkaP Mares
May 1, 1987·Cell and Tissue Research·R K Calverley, D G Jones
Apr 22, 1986·The Journal of Comparative Neurology·B J ClaiborneW M Cowan
Feb 1, 1995·Italian Journal of Neurological Sciences·E A Cavalheiro
Oct 1, 1996·Epilepsia·E A CavalheiroM R Priel
Dec 1, 1996·Epilepsy Research·M R PrielE A Cavalheiro
Jul 1, 1997·Biomaterials·J S Rees, P H Jacobsen
Oct 8, 1999·Clinical Nuclear Medicine·M Goyal, L G Hanelin
Apr 27, 2000·Nature Cell Biology·S Sankaranarayanan, T A Ryan
Mar 6, 2003·The Journal of Comparative Neurology·José E CavazosThomas P Sutula
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stefan HallermannManfred Heckmann
Dec 17, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Steve C Danzer, James O McNamara
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhiying LiVenkatesh N Murthy
Sep 9, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jérôme EpszteinValérie Crépel
Jun 24, 2006·Brain Research·Luis F Pacheco OtaloraEmilio R Garrido Sanabria
Jul 5, 2006·Cell and Tissue Research·Michael FrotscherRobert S Sloviter
Sep 21, 2006·The Annals of Occupational Hygiene·Chris Packham
Apr 20, 2007·Experimental Neurology·Karolien GoffinAsla Pitkänen
May 10, 2007·Nature Protocols·Josef BischofbergerPeter Jonas
Sep 4, 2007·Progress in Brain Research·F Edward Dudek, Thomas P Sutula
Sep 28, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Astrid RollenhagenJoachim H R Lübke
Oct 16, 2007·Neuroscience Research·Shigetomo SuyamaHiromu Yawo

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Citations

Sep 23, 2014·Orphanet Journal of Rare Diseases·Sylvia StocklerClara van Karnebeek
Dec 22, 2015·Molecular and Cellular Neurosciences·Tomohiko MaruoMasahiro Mori
Dec 19, 2012·Epilepsia·Pablo M Casillas-EspinosaTerence J O'Brien
Mar 18, 2015·Epilepsia·Kyle P LillisJokūbas Žiburkus
Mar 29, 2014·Frontiers in Molecular Neuroscience·Candi L Lasarge, Steve C Danzer
Oct 26, 2016·CNS Drugs·Wolfgang LöscherHenrik Klitgaard
Jun 12, 2020·Epilepsia Open·Pablo M Casillas-EspinosaTerence J O'Brien
Apr 13, 2021·Frontiers in Neurology·Suzanne LesageAlexis Brice
Jun 27, 2021·Journal of the Neurological Sciences·Nathan A ShlobinJosemir W Sander
Sep 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Aman B UpaganlawarTapan Behl

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