PMID: 1379Feb 1, 1976

Altered nutritional requirements associated with mutations affecting the structures of ribonucleic acid polymerase in Lactobacillus casei

Journal of Bacteriology
T Morishita, T Yura

Abstract

Rifampin-resistant mutants were isolated from Lactobacillus casei S1 and examined for possible simultaneous alteration in nutritional properties. Among the 36 mutants obtained either spontaneously or after mutagenesis with 2-aminopurine, 22 were found to be altered with respect to the specific growth requirements. The majority (20 of 22) of the latter mutants were shown to require L-glutamine in addition to the nutrients required by the parental strain for maximal growth, whereas the remaining mutants had apparently lost the requirement for L-aspartate. Further studies with one of the glutamine-requiring mutants revealed that the rifampin resistance of this strain is due to the resistance of ribonucleic acid polymerase itself and that a single mutation is responsible for both rifampin resistance and the glutamine requirement. These results strongly indicate that a structural alteration of the ribonucleic acid polymerase caused by the rifampin resistance mutation somehow affected glutamine metabolism, possibly through change in selective transcription of the genes involved.

Citations

Aug 15, 2014·The Journal of Antibiotics·Beth P Goldstein
May 3, 2000·Journal of Applied Microbiology·M ElliL Morelli

Related Concepts

Cell-Free System
Antibiotic Resistance, Microbial
Fermentation
D-Glutamine
Glutamate-Ammonia Ligase
Lactobacillus casei
Clastogens
Tubocin
Transcriptase
Molecular Stereochemistry

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