Altered oxygen tension modulates cytokine-induced signal transduction in polymorphonuclear leukocytes: regulation of the G PLC pathway

The Journal of Surgical Research
A DereviankoH Simms

Abstract

The purpose of these studies was to examine the sensitivity of the PIP 2-PLC-transducing pathway (GPLC) and its relationship to the respiratory burst in human polymorphonuclear leukocytes (PMN) stimulated by IL-8, TNF-alpha, or IL-1 beta during sequential changes in buffer oxygen tension from normoxia (pO2 = 180-200 mm Hg), to hypoxia (pO2 < 30 mm Hg) and then reoxygenation (pO2 > 140 mm Hg). Our specific hypothesis was that altered oxygen tensions would regulate the G PLC pathway in human PMN. G PLC activity was assayed by investigating phospholipase C activity by measuring inositol phosphates and diacylglycerol (DAG) formation. Respiratory burst activity was assayed as O 2 production and NADPH oxidase activation in intact PMN and in a cell-free system, respectively, and correlated separately to both early and late DAG production. At 1 min, DAG formation during normoxia was decreased by IL-8 plus fibronectin while hypoxia had no regulatory effect on control of DAG formation by any of the cytokines. In contrast to early DAG formation, hypoxia significantly downregulated late DAG formation induced by buffer without fibronectin, IL-8 plus fibronectin, and IL-1 beta with or without fibronectin. Hypoxia/reoxygenation in and of itse...Continue Reading

Citations

Feb 21, 2006·Biochimica Et Biophysica Acta·Mahefatiana AndrianifahananaSurinder K Batra
May 11, 2010·Free Radical Biology & Medicine·Corrine R Kliment, Tim D Oury
Jun 17, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M SprangerS Schwab
Dec 21, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenM Cemre Manav
Jun 9, 2005·American Journal of Physiology. Renal Physiology·Yifan ChenWilliam J Welch
Oct 22, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Vitalie FaoroHeimo Mairbäurl

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