Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein

Virology
I Leparc-GoffartS R Weiss

Abstract

C12, an attenuated, fusion delayed, very weakly hepatotropic mutant of mouse hepatitis virus strain A59 (MHV-A59( has been further characterized. We have previously shown that C12 has two amino acid substitutions relative to wild type virus in the spike protein, Q159L (within a region of S1 shown to bind to viral receptor in an in vitro assay) and H716D (in the proteolytic cleavage recognition site). We have sequenced the rest of the 31-kb genome of C-12 and compared it to wild type virus. Only three additional amino acids substitutions were found, all encoded within the replicase gene. Analysis of C12 in vivo in C57Bl/6 mice has shown that despite the fact that this virus replicates in the brain to titers at least as high as wild type and causes acute encephalitis similar to wild-type, this virus causes a minimal level of demyelination and only at very high levels of virus inoculation. Thus acute encephalitis is not sufficient for the induction of demyelination by MHV-A59. Analysis of mutants isolated at earlier times from the same persistently infected glial cell culture as C12, as well as mutants isolated from a second independent culture of persistently infected glial cells, suggests that both the weakly demyelinating and t...Continue Reading

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Citations

Apr 7, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Susan J Bender, Susan R Weiss
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guiqing PengFang Li
Jul 11, 2006·Journal of Clinical Microbiology·Akiko Yachi, Masami Mochizuki
Jul 15, 2006·Journal of Virology·X C TangY Guan
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Oct 12, 2012·Avian Diseases·Haroldo ToroMark W Jackwood
Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Feb 12, 2014·Archives of Virology·Ahmed S Abdel-Moneim
Jun 24, 2008·Avian Pathology : Journal of the W.V.P.A·Vicky L van Santen, Haroldo Toro
Aug 21, 2007·Virology·Jianqiang ZhangUdeni B R Balasuriya
Sep 1, 2016·Annual Review of Virology·Fang Li
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A E MatthewsY Paterson

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