Altered peptide ligands of myelin basic protein ( MBP87-99 ) conjugated to reduced mannan modulate immune responses in mice.

Immunology
Maria KatsaraVasso Apostolopoulos

Abstract

Mutations of peptides to generate altered peptide ligands, capable of switching immune responses from T helper 1 (Th1) to T helper 2 (Th2), are promising candidates for the immunotherapy of autoimmune diseases such as multiple sclerosis (MS). We synthesized two mutant peptides from myelin basic protein 87-99 (MBP(87-99)), an immunodominant peptide epitope identified in MS. Mutations of residues K(91) and P(96), known to be critical T-cell receptor (TCR) contact sites, resulted in the mutant peptides [R(91), A(96)]MBP(87-99) and [A(91), A(96)]MBP(87-99). Immunization of mice with these altered peptide ligands emulsified in complete Freund's adjuvant induced both interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) responses compared with only IFN-gamma responses induced to the native MBP(87-99) peptide. It was of interest that [R(91), A(96)]MBP(87-99) conjugated to reduced mannan induced 70% less IFN-gamma compared with the native MBP(87-99) peptide. However, [A(91), A(96)]MBP(87-99) conjugated to reduced mannan did not induce IFN-gamma-secreting T cells, but elicited very high levels of interleukin-4 (IL-4). Furthermore, antibodies generated to [A(91), A(96)]MBP(87-99) peptide conjugated to reduced mannan did not cross-react w...Continue Reading

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Citations

Dec 3, 2014·Cellular Immunology·Ameneh GhaffariniaNafiseh Pakravan
Apr 22, 2011·Expert Review of Vaccines·Nikolai Petrovsky, Peter D Cooper
Nov 15, 2013·Journal of Drug Delivery·Vasso ApostolopoulosLily Stojanovska
Jul 8, 2017·Brain Sciences·Narges DargahiVasso Apostolopoulos
Dec 4, 2014·Expert Review of Clinical Immunology·Nishi ModySuresh P Vyas
Dec 22, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emil R Unanue
May 15, 2020·Brain Sciences·Vasso ApostolopoulosJohn Matsoukas
Apr 22, 2017·Cancer Research·Anton SafonovLajos Pusztai
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vasso ApostolopoulosIstvan Toth
Nov 4, 2020·Brain Sciences·Vasso Apostolopoulos, John Matsoukas

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