Oct 18, 2005

Altered perlecan expression in placental development and gestational diabetes mellitus

Placenta
W-C V YangC-P Chen

Abstract

The proteoglycan perlecan is involved in cell signaling, regulation of growth factor activity, and maintenance of basement membranes. This study aims to investigate the expression of perlecan during placental development and whether hyperglycemia of gestational diabetes mellitus induces the alteration of perlecan expression in placenta. Immunohistochemistry, immunoprecipitation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantitative real-time PCR were carried out to study the placental perlecan expression at different trimesters of pregnancies and in gestational diabetes mellitus. The perlecan protein was mainly immunolocalized in the trophoblast and vessel basement membranes with some staining in the villous stroma of placental villus. Perlecan was also found to co-localize with laminin and collagen IV in the basement membranes of placenta. The protein and mRNA levels of placental perlecan were significantly decreased as the gestational age increased. However, a significant increase in perlecan expression was observed in the third trimester placentas with gestational diabetes mellitus compared to the gestational age-matched controls. Furthermore, trophoblast cells cultured in a high glucose (30 mM) medium a...Continue Reading

Mentioned in this Paper

Metabolic Process, Cellular
Real-Time Polymerase Chain Reaction
Pathogenic Aspects
Pathogenesis
Immunohistochemistry
Heparin
Trophoblast Cell
Immunoreactivity
Placentation
Syncytiotrophoblasts

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