PMID: 2115171Jul 1, 1990Paper

Altered phenotypic expression of immunoglobulin heavy-chain variable-region (VH) genes in Alicia rabbits probably reflects a small deletion in the VH genes closest to the joining region

Proceedings of the National Academy of Sciences of the United States of America
M AllegrucciR G Mage

Abstract

Rabbits of the Alicia strain have a mutation (ali) that segregates with the immunoglobulin heavy-chain (lgh) locus and has a cis effect upon the expression of heavy-chain variable-region (VH) genes encoding the a2 allotype. In heterozygous a1/ali or a3/ali rabbits, serum immunoglobulins are almost entirely the products of the normal a1 or a3 allele and only traces of a2 immunoglobulin are detectable. Adult homozygous ali/ali rabbits likewise have normal immunoglobulin levels resulting from increased production of a-negative immunoglobulins and some residual ability to produce the a2 allotype. By contrast, the majority of the immunoglobulins of wild-type a2 rabbits are a2-positive and only a small percentage are a-negative. Genomic DNAs from homozygous mutant and wild-type animals were indistinguishable by Southern analyses using a variety of restriction enzyme digests and lgh probes. However, when digests with infrequently cutting enzymes were analyzed by transverse alternating-field electrophoresis, the ali DNA fragments were 10-15 kilobases smaller than the wild type. These fragments hybridized to probes both for VH and for a region of DNA a few kilobases downstream of the VH genes nearest the joining region. We suggest that ...Continue Reading

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Citations

Apr 1, 1993·Current Opinion in Immunology·A Rolink, F Melchers
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·R PospisilR G Mage
Nov 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Guibin YangRose G Mage
Sep 25, 2015·Immunogenetics·Ana PinheiroPedro José Esteves
Dec 27, 2005·Developmental and Comparative Immunology·Richard PospisilRose G Mage
Aug 16, 2005·Developmental and Comparative Immunology·Rose G MageKatherine L Knight
Oct 1, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregory R Robbins, Katherine L Knight

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