Altered pHi regulation in 3T3/CFTR clones and their chemotherapeutic drug-selected derivatives

The American Journal of Physiology
L Y WeiP D Roepe

Abstract

Recently (L. Y. Wei, M. J. Stutts, M. M. Hoffman, and P. D. Roepe. Biophys. J. 69: 883-895, 1996), 3T3 cells overexpressing the cystic fibrosis transmembrane conductance regulator (CFTR) were found to exhibit chemotherapeutic drug resistance and other traits of multidrug resistant (MDR) cells. In the present work, NIH 3T3/CFTR clones were selected with either doxorubicin or vincristine in incremental fashion to generate series of stable MDR cell lines that exhibit increasing levels of drug resistance. Thus C3D6 (grown in the presence of 600 nM doxorubicin) was selected from C3D4 (grown in the presence of 400 nM doxorubicin), which was selected from C3D1 (grown in the presence of 100 nM doxorubicin), which was in turn selected from the original 3T3/CFTR clone C3 (M. J. Stutts, S. E. Gabriel, J. C. Olsen, J. T. Gatzy, T. L. O'Connell, E. M. Price, and R. C. Boucher. J. Biol. Chem. 268: 20653-20658, 1993), which was not grown in the presence of chemotherapeutic drug. A similar series was generated via selection with vincristine. In both series, as well as series derived from a different CFTR clone, initial low-level drug selection increases CFTR expression without promoting MDR 1 or multidrug resistance-associated protein expressi...Continue Reading

References

Apr 15, 1994·Biochemical and Biophysical Research Communications·C E Bear
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan

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Citations

Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M M MoralesA G Lopes
Apr 10, 1999·The Journal of Biological Chemistry·J H WeisburgD A Scheinberg
Jan 5, 2002·Physiological Reviews·Karl Kunzelmann, Marcus Mall
Oct 24, 1998·The Journal of Membrane Biology·P D Roepe
May 11, 1999·Drug Discovery Today·S M Simon
Apr 2, 1999·Trends in Pharmacological Sciences·P D Roepe, J A Martiney
Jun 15, 2004·Biochemical Pharmacology·Anca Dragomir, Godfried M Roomans

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