Altered placental expression of PAPPA2 does not affect birth weight in mice.

Reproductive Biology and Endocrinology : RB&E
Pamela K Wagner, Julian K Christians

Abstract

Pregnancy-associated plasma protein A2 (PAPPA2) is an insulin-like growth factor binding protein (IGFBP) protease expressed in the placenta and upregulated in pregnancies complicated by pre-eclampsia. The mechanism linking PAPPA2 expression and pre-eclampsia and the consequences of altered PAPPA2 expression remain unknown. We previously identified PAPPA2 as a candidate gene for a quantitative trait locus (QTL) affecting growth in mice and in the present study examined whether this QTL affects placental PAPPA2 expression and, in turn, placental or embryonic growth. Using a line of mice that are genetically homogenous apart from a 1 megabase QTL region containing the PAPPA2 gene, we bred mice homozygous for alternate QTL genotypes and collected and weighed placentae and embryos at E12.5. We used quantitative RT-PCR to measure the mRNA levels of PAPPA2, as well as mRNA levels of IGFBP-5 (PAPPA2's substrate), and PAPPA (a closely related IGFBP protease) to examine potential feedback and compensation effects. Western blotting was used to quantify PAPPA2 protein. Birth weight was measured in pregnancies allowed to proceed to parturition. PAPPA2 mRNA and protein expression levels in the placenta differed by a factor of 2.5 between gen...Continue Reading

References

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Sep 23, 2008·Molecular Human Reproduction·Haruki NishizawaYasuhiro Udagawa
May 29, 2009·The Journal of Endocrinology·Joyce WangJulian K Christians

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Citations

Apr 19, 2011·Reproductive Biology and Endocrinology : RB&E·Pamela K WagnerJulian K Christians
Sep 30, 2015·Reproductive Biology and Endocrinology : RB&E·Julian K ChristiansSonia M Hessels
Jul 15, 2015·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Julian K ChristiansNeilab Amiri
Dec 6, 2014·Reproductive Biology and Endocrinology : RB&E·Erin J CrosleyJulian K Christians
Mar 15, 2013·Reproduction, Fertility, and Development·Kate MacintireTu'uhevaha J Kaitu'u-Lino
Mar 14, 2018·Molecular Medicine Reports·Stavros SifakisNikos Papantoniou

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

Odyssey
JMP

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