PMID: 9434801Jan 22, 1998Paper

Altered postreceptor signal transduction of formyl-Met-Leu-Phe receptors in polymorphonuclear leukocytes of patients with non-insulin-dependent diabetes mellitus

Clinical Immunology and Immunopathology
G FórisJ Szabó

Abstract

The signal transduction of the formyl-Met-Leu-Phe (FMLP) receptor in polymorphonuclear leukocytes (PMNLs) from patients with non-insulin-dependent diabetes mellitus (NIDDM) was compared to that of PMNLs obtained from healthy volunteers. According to our previous studies in this group of patients neither the decrease in insulin binding capacity nor the enhanced insulin-degrading enzyme activity was involved. In control PMNLs, 10 nM FMLP induced a pertussis toxin-sensitive increase in phosphatidyl inositol (PI) cleavage and a subsequent Ca2+ signaling from the intracellular pools. On the other hand, the FMLP-induced protein kinase C (PKC) activation and translocation into the membrane could not be detected in these cells via the measurement of 32P incorporation into histone. In contrast, in PMNLs of this special group of patients suffering from NIDDM the FMLP stimulus produced a significantly low increase in PI cleavage and Ca2+ signaling from the intracellular pools. Moreover, in resting PMNLs of these patients with NIDDM, not only the [Ca2+]i but also the membrane-bound PKC activity was found to be significantly increased. In addition, PKC translocation into the cell membrane of diabetic PMNLs could be further triggered with FM...Continue Reading

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Citations

Aug 7, 2012·Neuropeptides·János Tamás PadraGyörgy Paragh
Feb 27, 2004·European Journal of Clinical Investigation·A AdvaniT H Thomas
Feb 10, 2004·Biochemical and Biophysical Research Communications·Abbas E KitabchiGuillermo E Umpierrez
Aug 30, 2005·Metabolism: Clinical and Experimental·Ildiko SeresGyorgy Paragh

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