Abstract
Cyclodextrin glycosyltransferases (CGTases), members of glycoside hydrolase family 13, catalyze the conversion of amylose to cyclodextrins (CDs), circular alpha-(1,4)-linked glucopyranose oligosaccharides of different ring sizes. The CD containing 12 alpha-D-glucopyranose residues was preferentially synthesized by molecular imprinting of CGTase from Paenibacillus sp. A11 with cyclomaltododecaose (CD(12)) as the template molecule. The imprinted CGTase was stabilized by cross-linking of the derivatized protein. A high proportion of CD(12) and larger CDs was obtained with the imprinted enzyme in an aqueous medium. The molecular imprinted CGTase showed an increased catalytic efficiency of the CD(12)-forming cyclization reaction, while decreased k(cat)/K(m) values of the reverse ring-opening reaction were observed. The maximum yield of CD(12) was obtained when the imprinted CGTase was reacted with amylose at 40 degrees C for 30 min. Molecular imprinting proved to be an effective means toward increase in the yield of large-ring CDs of a specific size in the biocatalytic production of these interesting novel host compounds for molecular encapsulations.
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Mar 1, 1966·Analytical Biochemistry·A F Habeeb
Sep 1, 1973·The Analyst·R J HallD I Givens
Jul 1, 1965·Archives of Biochemistry and Biophysics·D FrenchM Abdullah
Jun 20, 1997·The Journal of Biological Chemistry·Y TeradaS Okada
Dec 1, 1999·Bioorganic & Medicinal Chemistry·F Peissker, L Fischer
Apr 3, 2001·Applied and Environmental Microbiology·Y TeradaS Okada
Apr 5, 2001·Proteins·J C UitdehaagB W Dijkstra
Feb 19, 2002·Chemical Reviews·Wolfram SaengerTakeshi Takaha
May 15, 1961·Biochemical and Biophysical Research Communications·A O PULLEY, D FRENCH
Dec 26, 2003·Chembiochem : a European Journal of Chemical Biology·Alankar VaidyaLutz Fischer
Aug 27, 2004·Biotechnology and Bioengineering·R SchoevaartR A Sheldon
Jan 5, 2005·Applied Microbiology and Biotechnology·Qingsheng Qi, Wolfgang Zimmermann
Jan 26, 2007·The FEBS Journal·Jarunee KaulpiboonWolfgang Zimmermann
Oct 17, 2008·Carbohydrate Research·Hernán CostaMirtha Biscoglio de Jiménez Bonino
Apr 16, 2009·Applied Microbiology and Biotechnology·Ronan M KellyHans Leemhuis
Citations
Mar 25, 2011·Journal of Industrial Microbiology & Biotechnology·Quanfeng LiangQingsheng Qi
Dec 15, 2015·Biotechnology Advances·Zofia IskierkoWlodzimierz Kutner
Dec 24, 2013·Biotechnology Advances·Ruizhi HanJian Chen
Oct 26, 2018·MicrobiologyOpen·Christian SonnendeckerWolfgang Zimmermann
Nov 28, 2012·Chemistry : a European Journal·Renat Kadyrov
Jul 2, 2017·3 Biotech·Yi-Nan YangPi-Wu Wang