Altered protein conformation on DNA binding by Fos and Jun

Nature
L R PatelT Curran

Abstract

The protein products of the c-fos and c-jun proto-oncogenes (Fos and Jun, respectively) form a heterodimeric protein complex that interacts with the activator protein-1 (AP-1) binding site and regulates gene transcription in response to extracellular stimuli. Protein dimerization is mediated primarily by a coiled-coil-like structure termed the leucine-zipper and DNA binding occurs primarily through regions of each protein rich in basic amino acids that contact both strands of the AP-1 site. The precise nature of the protein-DNA interaction is unknown as studies concerned with dimerization and DNA binding by Fos and Jun have relied on indirect methods to investigate protein-protein-DNA interactions. Here we have developed assay systems using fluorescence spectroscopy and circular dichroism to monitor dimerization and DNA binding directly. The results indicate that the interaction of Fos and Jun with DNA results in an altered conformation of the protein dimers and an increased alpha-helical content. These techniques may have general application in studies concerning the interaction of transcriptional regulatory proteins with specific DNA target sequences.

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C AbateT Curran
Oct 1, 1990·Molecular and Cellular Biology·C AbateT Curran
Dec 1, 1988·Genes & Development·F J RauscherT Curran
Jan 27, 1989·Science·E K O'SheaP S Kim
Dec 15, 1988·Nature·T Kouzarides, E Ziff
Oct 20, 1988·Nature·M Ptashne
Nov 4, 1988·Cell·T Curran, B R Franza

❮ Previous
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Citations

Feb 1, 1993·Journal of Cellular Biochemistry·L P Freedman, B F Luisi
Sep 1, 1993·Protein Science : a Publication of the Protein Society·Z I Santiago-RiveraO M Andrisani
Mar 1, 1995·Protein Science : a Publication of the Protein Society·S Udenfriend
Sep 23, 1993·Biochimica Et Biophysica Acta·K A Lee, N Masson
Jun 1, 1991·Current Opinion in Cell Biology·M Carey
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·K Dahlman-WrightJ A Gustafsson
Jul 1, 1993·Trends in Cell Biology·R Y TsienS R Adams
Jun 1, 2006·Biochemistry·Jiangang LiuA Keith Dunker
Apr 22, 2010·Biochemistry·Rajarshi P GhoshChristopher L Woodcock
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Jun 1, 1994·Nature Structural Biology·B R SinghD N Ledoux
Jun 1, 1995·Nature Structural Biology·D Stanojevic, G L Verdine
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T Deng, M Karin
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D TzamariasK Struhl
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J KimK Struhl
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C ParkW A Goddard
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C AbateT Curran
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·L R PatelT K Kerppola
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·W Du, T Maniatis
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·J C BeckerR A Reisfeld
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S Williams, O M Andrisani
May 24, 2008·The Journal of Biological Chemistry·Rajarshi P GhoshChristopher L Woodcock
Jul 25, 1992·Nucleic Acids Research·W J van HeeckerenK Struhl
Sep 25, 1993·Nucleic Acids Research·R T Hay, J Nicholson
Feb 12, 1998·Nucleic Acids Research·D MeierhansR K Allemann
Sep 26, 1997·Genes & Development·A S CarrollT K Blackwell
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Jessica J HollenbeckMartha G Oakley
Aug 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M WonH S Yoo
Feb 23, 2013·Journal of Bioinformatics and Computational Biology·Edgar Wingender
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·S PalS Walker
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·J Vicente-CarbajosaR J Schmidt
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A LeonardT K Kerppola
Feb 1, 1994·Trends in Biochemical Sciences·T G Oas, S A Endow

❮ Previous
Next ❯

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