Altered regulation of renal Acid base transporters in response to ammonium chloride loading in rats.

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Eun Young KimSoo Wan Kim

Abstract

The role of the kidney in combating metabolic acidosis has been a subject of considerable interest for many years. The present study was aimed to determine whether there is an altered regulation of renal acid base transporters in acute and chronic acid loading. Male Sprague-Dawley rats were used. Metabolic acidosis was induced by administration of NH(4)Cl for 2 days (acute) and for 7days (chronic). The serum and urinary pH and bicarbonate were measured. The protein expression of renal acid base transporters [type 3 Na(+)/H(+) exchanger (NHE3), type 1 Na(+)/HCO(3) (-) cotransporter (NBC1), Na-K(+) ATPase, H(+)-ATPase, anion exchanger-1 (AE-1)] was measured by semiquantitative immunoblotting. Serum bicarbonate and pH were decreased in acute acid loading rats compared with controls. Accordingly, urinary pH decreased. The protein expression of NHE3, H(+)-ATPase, AE-1 and NBC1 was not changed. In chronic acid loading rats, serum bicarbonate and pH were not changed, while urinary pH was decreased compared with controls. The protein expression of NHE3, H(+)-ATPase was increased in the renal cortex of chronic acid loading rats. These results suggest that unaltered expression of acid transporters combined with acute acid loading may con...Continue Reading

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L AlperD Brown
Dec 1, 1985·Pflügers Archiv : European journal of physiology·K YoshitomiE Frömter
Jan 1, 1980·Kidney International·L GoldsteinH Schröck
Jan 1, 1982·The Journal of Clinical Investigation·A TizianelloG M Ghiggeri
Apr 1, 1999·Annual Review of Physiology·M F Romero, W F Boron
Sep 1, 2000·American Journal of Physiology. Renal Physiology·G H KimM A Knepper
Aug 15, 2001·American Journal of Physiology. Renal Physiology·N P Curthoys, G Gstraunthaler
Jan 15, 2002·American Journal of Physiology. Renal Physiology·Tae-Hwan KwonSøren Nielsen
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Young-Hee KimSøren Nielsen
Jul 28, 2005·Current Opinion in Nephrology and Hypertension·Susan M Wall
Jul 27, 2006·Physiological Genomics·Lydie ChevalAlain Doucet
Oct 31, 2006·Seminars in Nephrology·I Alexandru Bobulescu, Orson W Moe
Dec 7, 2007·Physiological Genomics·Marta NowikCarsten A Wagner
Jun 7, 2008·Pflügers Archiv : European journal of physiology·Marta NowikCarsten A Wagner
Oct 31, 2008·American Journal of Physiology. Cell Physiology·Patricia HafnerCarsten A Wagner
Mar 12, 2009·Pflügers Archiv : European journal of physiology·Carsten A WagnerNilufar Mohebbi
Dec 1, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Eun Hui BaeSoo Wan Kim

❮ Previous
Next ❯

Citations

Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron

❮ Previous
Next ❯

Methods Mentioned

BETA
urine collections
electrophoresis

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Related Papers

Current Opinion in Critical Care
Lakhmir S Chawla
Current Opinion in Critical Care
Nattachai Srisawat, John A Kellum
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Hassane Amlal, Manoocher Soleimani
© 2021 Meta ULC. All rights reserved