PMID: 6112013Mar 3, 1981Paper

Altered regulation of the guanosine 5'-triphosphate activity in a kirromycin-resistant elongation factor Tu

Biochemistry
O Fasano, A Parmeggiani

Abstract

In the preceding article a mutant elongation factor Tu (EF-TuD2216) resistant to the action of kirromycin was found to display a spontaneous guanosine 5'-triphosphatase (GTPase) activity, i.e., in the absence of aminoacyl transfer ribonucleic acid (tRNA) and ribosome-messenger RNA. This is the first example of an Ef-Tu supporting GTPase activity in the absence of macromolecular effectors and/or kirromycin. In this study we show that this activity is elicited by increasing NH4+ concentrations. As additional effect, the mutation caused an increased affinity of EF-Tu for GTP. Ammonium dependence of the GTPase activity an increased affinity for GTP are two properties also found with wild-type EF-Tu in the presence of kirromycin [Fasano, O., Burns, W., Crechet, J.-B., Sander, G., & Parmeggiani, A. (1978) Eur. J. Biochem. 89, 557-565; Sander, G., Okonek, M., Crechet, J.-B., Ivell, R., Bocchini, V., & Parmeggiani, A. (1979) FEBS Lett. 98, 111-114]. Therefore, both binding of kirromycin to wild-type EF-Tu and acquisition of kirromycin resistance introduce functionally related modifications. Kirromycin at high concentrations (0.1 mM) does not interact with mutant EF-TuD2216.GDP but still does with EF-TuD2216.GTP in agreement with our pr...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R C Thompson, P J Stone
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E FischerA Parmeggiani
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H WolfA Parmeggiani
Nov 1, 1970·Archives of Biochemistry and Biophysics·D L Miller, H Weissbach
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J DAVIESL GORINI

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Citations

Mar 27, 1981·Molecular and Cellular Biochemistry·A Parmeggiani, G Sander
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V ManneH F Kung
Mar 10, 1990·European Journal of Biochemistry·S Tapio, L A Isaksson
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B KraalR Hilgenfeld
Jan 10, 1989·Biochemistry·G W Swart, A Parmeggiani
Feb 15, 1992·European Journal of Biochemistry·M Y MistouA Parmeggiani

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