Altered renal morphology in transgenic mice with cholecystokinin overexpression.

Transgenic Research
Marina AunapuuAndres Arend

Abstract

Although cholecystokinin is a regulatory peptide with a predominant role in the brain and the gastrointestinal tract, there is an increasing evidence for its role in the kidney. The aim of this study was to reveal morphological changes in the structure of kidney of mice with cholecystokinin overexpression by means of light, transmission and scanning electron microscope, and atomic force microscopy. Using immunohistochemistry the expression of important basement membrane proteins collagen IV, laminin and fibronectin, as well the distribution of cholecystokinin-8 in the renal structures was evaluated. The altered morphology of kidneys of mice with cholecystokinin overexpression was seen by all microscopic techniques used. The renal corpuscles were relatively small with narrow capsular lumen. The basement membranes of renal tubules were thickened and the epithelial cells were damaged, which was more pronounced for distal tubules. Characteristic feature was the increased number of vesicles seen throughout the epithelial cells of proximal and especially in distal tubules reflecting to the enhanced cellular degeneration. The relative expression of laminin but not collagen IV in the glomerular basement membrane was higher than in the ...Continue Reading

References

Sep 1, 1987·The British Journal of Nutrition·J CalamC J Springer
Oct 1, 1983·Neuropeptides·M C Beinfeld
Jan 1, 1995·Renal Physiology and Biochemistry·D K BreesJ C Williams
Dec 14, 1999·Kidney International·J H Miner
Jun 22, 2000·Biochemical and Biophysical Research Communications·J M LayL C Samuelson
Feb 22, 2002·European Journal of Biochemistry·Takako SasakiRupert Timpl
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Tetsuya ShigeharaJeffrey B Kopp
Sep 16, 2003·Nephron. Experimental Nephrology·Jeffrey H Miner
Feb 14, 2004·Biophysical Chemistry·Nuno C Santos, Miguel A R B Castanho
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Jeffrey H Miner, Peter D Yurchenco
Aug 4, 2006·The Journal of Clinical Investigation·Marilyn G Farquhar
Sep 8, 2006·Kidney International·S V Dandapani, M R Pollak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.