PMID: 3084104Apr 25, 1986

Altered repertoire of endogenous immunoglobulin gene expression in transgenic mice containing a rearranged mu heavy chain gene

Cell
D WeaverT Imanishi-Kari

Abstract

C57BL/6 mice transgenic for a mu heavy chain gene, the VDJ region of which came from the BALB/c hybridoma 17.2.25, expressed high levels of antibody carrying determinants specific for the transgene (idiotypes). The individual antibodies made by hybridomas from transgenic mice, however, were generally encoded by endogenous genes; in most cases the transgene was present but not expressed. The endogenous, idiotype-positive antibodies had heavy chains that were notable for the high frequencies of JH4 (as in the transgene) and VH segments from the VH81X family (unrelated to the transgene). The expression of endogenous genes mimicking the idiotype of the transgene suggests that a rearranged gene introduced into the germ line can activate powerful cellular regulatory influences.

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Related Concepts

Monoclonal Antibodies
DNA, Double-Stranded
Hybridomas
Immunoglobulin Idiotypes
Immunoglobulin Heavy Chain Subgroup VH-III
Immunoglobulin mu-Chains
Mice, Inbred BALB C
Mice, Inbred C57BL
Microinjections
Proteins, Recombinant DNA

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