Altered seed oil and glucosinolate levels in transgenic plants overexpressing the Brassica napus SHOOTMERISTEMLESS gene

Journal of Experimental Botany
Mohamed ElhitiClaudio Stasolla

Abstract

SHOOTMERISTEMLESS (STM) is a homeobox gene conserved among plant species which is required for the formation and maintenance of the shoot meristem by suppressing differentiation and maintaining an undetermined cell fate within the apical pole. To assess further the role of this gene during seed storage accumulation, transgenic Brassica napus (Bn) plants overexpressing or down-regulating BnSTM under the control of the 35S promoter were generated. Overexpression of BnSTM increased seed oil content without affecting the protein and sucrose level. These changes were accompanied by the induction of genes encoding several transcription factors promoting fatty acid (FA) synthesis: LEAFY COTYLEDON1 (BnLEC1), BnLEC2, and WRINKLE1 (BnWRI1). In addition, expression of key representative enzymes involved in sucrose metabolism, glycolysis, and FA biosynthesis was up-regulated in developing seeds ectopically expressing BnSTM. These distinctive expression patterns support the view of an increased carbon flux to the FA biosynthetic pathway in developing transformed seeds. The overexpression of BnSTM also resulted in a desirable reduction of seed glucosinolate (GLS) levels ascribed to a transcriptional repression of key enzymes participating in...Continue Reading

References

May 1, 1992·Plant Molecular Biology·A R Slabas, T Fawcett
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Nov 1, 1994·Plant Physiology·P BergmanK Glimelius
Mar 9, 2000·Trends in Plant Science·A A MillarL Kunst
Jan 5, 2002·Progress in Lipid Research·Stephen Rawsthorne
Jan 22, 2002·Metabolic Engineering·Jay J Thelen, John B Ohlrogge
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 22, 2003·The Journal of Biological Chemistry·Jorg SchwenderYair Shachar-Hill
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Sébastien BaudChristine Rochat
Jan 13, 2004·BMJ : British Medical Journal·Adrian TowseViroj Tangcharoensathien
May 12, 2004·Current Opinion in Plant Biology·Matthew J Hills
Oct 7, 2004·Plant Physiology·Douglas H HobbsMatthew J Hills
Sep 6, 2005·Current Biology : CB·Osnat YanaiNaomi Ori
Jan 13, 2006·Trends in Plant Science·C Douglas Grubb, Steffen Abel
May 26, 2006·Plant Molecular Biology·Simon Scofield, James A H Murray
Jun 20, 2006·Autoimmunity Reviews·Anna Abou-Raya, Suzan Abou-Raya
Feb 9, 2007·Nature·Takashi KurakawaJunko Kyozuka
Apr 11, 2007·The Plant Journal : for Cell and Molecular Biology·Sébastien BaudBertrand Dubreucq
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Naoyuki UchidaNeelima Sinha
Feb 16, 2008·Nature Protocols·Prem L Bhalla, Mohan B Singh
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sandra L StoneJohn J Harada

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Citations

Jan 17, 2016·Plant Physiology and Biochemistry : PPB·Nosheen ElahiClaudio Stasolla
Aug 25, 2015·Plant Physiology and Biochemistry : PPB·Nosheen ElahiClaudio Stasolla
Oct 4, 2016·Scientific Reports·Yu-Qin ZhangJin-Song Zhang
Jun 1, 2016·Journal, Genetic Engineering & Biotechnology·Nosheen ElahiClaudio Stasolla
Oct 22, 2020·Plant Signaling & Behavior·Tingting ZhuMin Chen

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