Altered sensitivity to retinoid-induced apoptosis associated with changes in the subcellular distribution of Bcl-2

Experimental Cell Research
A BruelM Lanotte

Abstract

In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We observed that NB4 and NB4-R1 cells differed with respect to the intracellular localization of Bcl-2 which showed a perinuclear localization in NB4-R1 cells, while Bax was broadly expressed in the cytoplasm and to only a minor extent in the perinuclear area. Therefore, the distinct intracellular localization of Bcl-2 and Bax was in general nonoverlapping. Bcl-2 remained massively expressed until cell disruption. Bax was not significantly upregulated in cells committed to death. However, Bax localization changed from a diffuse pattern to concentrate in few specific cytoplasmic area at a stage preceding the formation of apoptotic bodies. A human Bcl-2 transgene was transiently overexpressed in NB4-R1 cells which showed increased resistance to apoptosis induced by retinoids. Stably transfected clones of NB4-R1 cells showed an increased expression of Bcl-2 and a marked resistance to apoptosis. Interestingly, the overexpr...Continue Reading

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Citations

Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D W VoehringerL A Herzenberg
May 6, 2015·Seminars in Cancer Biology·Ramzi M MohammadAsfar S Azmi
Nov 9, 2000·The British Journal of Dermatology·T C IslamR Toftgård
Apr 25, 2000·Cell Death and Differentiation·R HoetelmansJ H Dierendonck
Aug 19, 2004·Journal of Biomedical Science·Chung-Liang ChienKuo-Shyan Lu
Apr 27, 1999·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·P P KotyM L Levitt

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