Altered susceptibility to apoptosis and N‑glycan profiles of hematopoietic KG1a cells following co‑culture with bone marrow‑derived stromal cells under hypoxic conditions

Oncology Reports
Xingchen PangXiang Li

Abstract

Mesenchymal stromal cells are an important component of the bone marrow microenvironment (niche), where they support hematopoiesis via direct cell‑cell interactions with hematopoietic stem and progenitor cells, and by releasing soluble factors. Glycans, including N‑glycans, are involved in numerous biological processes, including inflammation, cell‑cell interactions, as well as cancer development and progression. Lectin‑based microarray analysis has provided a powerful new tool in recent years, for the investigation of aberrantly expressed N‑glycans and their functions in the bone marrow microenvironment. In the present study, we used an in vitro stromal/hematopoietic cell co‑culture system to examine the effects of stromal‑derived signals on apoptosis susceptibility of co‑cultured KG1a hematopoietic cells under hypoxic (1% O2) conditions. MALDI‑TOF/TOF‑MS analysis was used for the comparative global profiling of N‑glycans in KG1a cells and co‑cultured KG1a cells under hypoxia. KG1a cells became more susceptible to p53‑dependent apoptosis when co‑cultured with HS27A human stromal cells (derived from normal bone marrow) under hypoxia. We observed enhanced levels of core‑fucosylated N‑glycans (catalyzed by FUT8), bisecting GlcNAc...Continue Reading

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.