Altered thyrotropin (TSH) carbohydrate structures in hypothalamic hypothyroidism created by paraventricular nuclear lesions are corrected by in vivo TSH-releasing hormone administration

Endocrinology
T Taylor, B D Weintraub

Abstract

TSH is a glycoprotein hormone composed of two subunits with attached carbohydrate chains that have varying structural characteristics. To determine the role of TRH in vivo in regulating structural characteristics of TSH carbohydrate chains, adult rats received paraventricular nuclear (PVN) lesions (n = 6) or sham lesions (n = 6). The PVN contain large amounts of TRH, and rats with lesions in these hypothalamic nuclei have been shown to have decreased plasma thyroid hormone levels. At 10 days after surgery, sc osmotic pumps infusing saline or 1 mg/kg/day TRH were placed. At 14 days after surgery, pituitaries were removed and incubated with [3H]glucosamine for 24 h. Glycopeptides prepared from secreted TSH were sequentially eluted from Concanavalin-A chromatography columns selecting unbound, weakly bound, and strongly bound forms. Plasma free T4 was lower in the PVN lesioned rats treated with saline than sham lesioned rats treated with saline (1.6 +/- 0.4 vs. 5.2 +/- 0.1 ng/dl, P less than 0.001). In vivo TRH administration in the PVN lesioned group normalized plasma free T4 but had no effect on free T4 in the sham group. Secreted TSH glycopeptides in the PVN lesioned rats treated with saline as compared to sham lesioned rats tre...Continue Reading

Citations

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