PMID: 7035818Jan 1, 1981Paper

Altered transcriptional termination in a rifampicin-resistant mutant of Escherichia coli which inhibits the growth of bacteriophage T7

Molecular & General Genetics : MGG
T F SchwarzD J McConnell

Abstract

A spontaneous rifampicin-resistant mutant of E. coli K12, RpoB26, which inhibits the growth of bacteriophage T7 has been isolated. The mutation is an RNA polymerase mutation; it also restores the wild-type effect of polar mutations in a rho-deficient strain, probably by restoring transcriptional termination. The efficiency of plating (e.o.p.) of wild-type T7, and of some early region deletion and point mutants of T7 tested, is reduced on RpoB26 by a factor of 10(-4). However, some deletion mutants are inhibited more severely (up to 10(-7) on RpoB26. We argue that these differences may reflect variations in the frequency of transcriptional termination before gene 1, an essential gene which codes for the T7 RNA polymerase (Summers and Siegel 1970; Chamberlin et al. 1970). We also present data which suggest that the product of a late T7 gene plays a role, by some interaction with the product of gene 1, in the inhibition of T7 in RpoB26. We suggest that different levels of expression of gene 1 may lead to different degrees of inhibition of T7 strains in RpoB26.

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·S M YeatsD J McConnell
Jul 29, 2005·Antimicrobial Agents and Chemotherapy·Véronique DartoisThomas R Parr
Oct 3, 2012·Evolutionary Applications·Patricia Escobar-PáramoMichael E Hochberg
Feb 24, 2001·International Journal of Clinical & Laboratory Research·L PuccettiA L Pasqui
Jan 1, 1982·Molecular & General Genetics : MGG·S R Buchstein, D C Hinkle

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