Altered wound healing in mice lacking a functional osteopontin gene (spp1)

The Journal of Clinical Investigation
L LiawB L Hogan

Abstract

Osteopontin (OPN) is an arginine-glycine-aspartate (RGD)- containing glycoprotein encoded by the gene secreted phosphoprotein 1 (spp1). spp1 is expressed during embryogenesis, wound healing, and tumorigenesis; however, its in vivo functions are not well understood. Therefore, OPN null mutant mice were generated by targeted mutagenesis in embryonic stem cells. In OPN mutant mice, embryogenesis occurred normally, and mice were fertile. Since OPN shares receptors with vitronectin (VN), we tested for compensation by creating mice lacking both OPN and VN. The double mutants were also viable, suggesting that other RGD-containing ligands replace the embryonic loss of both proteins. We tested the healing of OPN mutants after skin incisions, where spp1 was upregulated as early as 6 h after wounding. Although the tensile properties of the wounds were unchanged, ultrastructural analysis showed a significantly decreased level of debridement, greater disorganization of matrix, and an alteration of collagen fibrillogenesis leading to small diameter collagen fibrils in the OPN mutant mice. These data indicate a role for OPN in tissue remodeling in vivo, and suggest physiological functions during matrix reorganization after injury.

References

Jul 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·J N CloreR F Diegelmann
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A OldbergD Heinegård
Aug 7, 1980·Nature·D R SengerR O Hynes
Apr 21, 1995·Annals of the New York Academy of Sciences·D T DenhardtS E Walther
Mar 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D E BirkE I Zycband
Apr 21, 1995·Annals of the New York Academy of Sciences·B B MukherjeeA B Mukherjee
Apr 21, 1995·Annals of the New York Academy of Sciences·W T Butler
Jun 15, 1995·The Journal of Experimental Zoology·J M ThayerS M Schwartz
May 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L B RoweE H Birkenmeier
Feb 1, 1994·Kidney International·C M GiachelliR J Johnson
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·X ZhengD Ginsburg
Oct 1, 1996·Cytokine & Growth Factor Reviews·G F Weber, H Cantor
Feb 10, 1997·The Journal of Cell Biology·K G DanielsonR V Iozzo
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·X LiuR Jaenisch
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D E BirkR L Trelstad
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·R V Iozzo

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Citations

Mar 24, 1999·Journal of Cellular Biochemistry·L L SmithC M Giachelli
Feb 16, 2000·Biochemical and Biophysical Research Communications·S T BarryC M Horgan
Apr 7, 2005·Calcified Tissue International·Rachit OhriCecilia M Giachelli
Dec 17, 2008·Cancer Chemotherapy and Pharmacology·Flaubert Mbeunkui, Donald J Johann
May 7, 2008·Journal of the Association for Research in Otolaryngology : JARO·Xing ZhaoYunxia Wang Lundberg
Aug 3, 2005·Cellular and Molecular Neurobiology·Z N Akin, A J Nazarali
May 17, 2008·Journal of Materials Science. Materials in Medicine·Rupak M RajacharCecilia M Giachelli
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Yu-Hua HsiehPi-Ling Chang
Dec 8, 2010·Immunologic Research·Makoto Inoue, Mari L Shinohara
Mar 23, 2004·Biochemical and Biophysical Research Communications·Taeko IshiiYukihiko Saeki
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Kim S MidwoodJean E Schwarzbauer
Jun 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Sara M WeisAndrew D McCulloch
Apr 15, 2004·Molecular and Cellular Neurosciences·Raghuram SelvarajuUrsula Boschert
Nov 9, 2000·Immunology Today·A W O'ReganJ S Berman
Feb 5, 2002·Biochimica Et Biophysica Acta·G F Weber
Jan 4, 2001·Brain Research Bulletin·G F Weber, S Ashkar
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·C M Giachelli, S Steitz
Jul 3, 2003·Molecular and Cellular Neurosciences·Sherry T HikitaDennis O Clegg
Jun 27, 2003·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Issei KomatsubaraYasushi Shiratori
Dec 31, 2002·Journal of Diabetes and Its Complications·Sunao AsaumiSeijiro Mori
Oct 18, 2003·Cytokine & Growth Factor Reviews·Anthony O'Regan
Mar 6, 2003·Gene Expression Patterns : GEP·Douglas B Menke, David C Page
May 7, 2011·Expert Reviews in Molecular Medicine·Susan R Rittling
Oct 7, 2005·Leukemia·V Cheriyath, M A Hussein
Apr 12, 2001·International Journal of Experimental Pathology·A O'Regan, J S Berman
Aug 5, 1999·Kidney International·V OphascharoensukR J Johnson
Jun 26, 2002·Kidney International·Marilda MazzaliRichard J Johnson
Jun 2, 2005·The Journal of Experimental Medicine·Sebastian StierDavid T Scadden
Apr 29, 2011·European Heart Journal·Stelios PsarrasYassemi Capetanaki
May 15, 2007·International Journal of Epidemiology·Hyoung Doo ShinHyo-Suk Lee

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