Altering expression of the flavonoid 3'-hydroxylase gene modified flavonol ratios and pollen germination in transgenic Mitchell petunia plants

Functional Plant Biology : FPB
David H LewisKevin M Davies

Abstract

Antisense technology was successfully used to reduce flavonoid 3'-hydroxylase (F3'H) gene expression and enzyme activity and to promote the accumulation of monohydroxylated flavonols in petunia flower tissue. The hydroxylation pattern of specific flavonoid groups is a target for modification because of the possible associated changes in a range of factors including colour, stress tolerance and reproductive viability. Petunia (cv. Mitchell) plants were transformed to express in the antisense orientation the sequences encoding the F3'H (asF3'H). Transformants showed a range of responses, in terms of the level of endogenous F3'H gene expression and the relative proportion of the monohydroxylated flavonol (kaempferol) glycosides that accumulated. Kaempferol glycosides increased from 7% of the total flavonols in flower limb tissue of the wild type plants, to 45% in the flower limb tissue of line 114, the transgenic line that also showed the greatest decrease in F3'H expression in flower tissue. In leaf tissue, the trend was for a decrease in total flavonol concentration, with the relative proportion of kaempferol glycosides varying from ~40 to 80% of the total flavonols. The changes in leaf tissue were not consistent with the change...Continue Reading

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Methods Mentioned

BETA
transgenic
transgenics
glycosylation

Software Mentioned

Genstat

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