Alternate energy coupling of ArsB, the membrane subunit of the Ars anion-translocating ATPase.

The Journal of Biological Chemistry
M KurodaB P Rosen

Abstract

The arsenical resistance (ars) operon of the conjugative R-factor R773 confers resistance to arsenical and antimonial compounds in Escherichia coli, where resistance results from active extrusion of arsenite catalyzed by the products of the arsA and arsB genes. Previous in vivo studies on the energetics of arsenite extrusion showed that expression of both genes produced an ATP-coupled arsenite extrusion system that was independent of the electrochemical proton gradient. In contrast, in cells expressing only the arsB gene, arsenite extrusion was coupled to electrochemical energy and independent of ATP, suggesting that the Ars transport system exhibits a dual mode of energy coupling depending on the subunit composition. In vitro the ArsA-ArsB complex has been shown to catalyze ATP-coupled uptake of 73AsO2(-1) in everted membrane vesicles. However, transport catalyzed by ArsB alone has not previously been observed in vitro. In this study we demonstrate everted membrane vesicles prepared from cells expressing only arsB exhibit uptake of 73AsO2(-1) coupled to electrochemical energy.

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Citations

Oct 24, 2007·Journal of Bioenergetics and Biomembranes·Yung-Feng LinBarry P Rosen
May 12, 2001·FEMS Microbiology Reviews·C CervantesR Moreno-Sánchez
Aug 29, 2001·FEMS Microbiology Reviews·D J Kelly, G H Thomas
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Nov 22, 2011·The Journal of Biological Chemistry·Almudena F VilladangosLuis M Mateos
Oct 9, 2009·Journal of Cell Science·Catherine RabuStephen High
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Om Parkash DhankherRichard B Meagher
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zijuan LiuBarry P Rosen
Feb 11, 2009·Journal of Environmental Sciences (China)·Jin-Soo ChangKyoung-Woong Kim
Feb 19, 2010·The New Phytologist·Fang-Jie ZhaoJian Feng Ma
Jul 22, 2010·Biochimica Et Biophysica Acta·Nica Borgese, Elisa Fasana
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