Alternate Immersion in an External Glucose Solution Differentially Affects Blood Sugar Values in Older Versus Younger Zebrafish Adults

Zebrafish
Victoria P ConnaughtonCarly Slack

Abstract

Recently, zebrafish have been used to examine hyperglycemia-induced complications (retinopathy and neuropathy), as would occur in individuals with diabetes. Current models to induce hyperglycemia in zebrafish include glucose immersion and streptozotocin injections. Both are effective, although neither is reported to elevate blood sugar values for more than 1 month. In this article, we report differences in hyperglycemia induction and maintenance in young (4-11 months) versus old (1-3 years) zebrafish adults. In particular, older fish immersed in an alternating constant external glucose solution (2%) for 2 months displayed elevated blood glucose levels for the entire experimental duration. In contrast, younger adults displayed only transient hyperglycemia, suggesting the fish were acclimating to the glucose exposure protocol. However, modifying the immersion protocol to include a stepwise increasing glucose concentration (from 1% → 2%→3%) resulted in maintained hyperglycemia in younger zebrafish adults for up to 2 months. Glucose-exposed younger fish collected after 8 weeks of exposure also displayed a significant decrease in wet weight. Taken together, these data suggest different susceptibilities to hyperglycemia in older and ...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L ChenR H Unger
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L OrciR H Unger
Feb 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mary D Kinkel, Victoria E Prince
May 22, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yung-Che TsengPung-Pung Hwang
Jun 6, 2009·Diabetes·Jennifer B MossLarry G Moss
Feb 10, 2010·Disease Models & Mechanisms·Yolanda AlvarezBreandán N Kennedy
Jun 3, 2010·Zebrafish·Stefani C EamesMary D Kinkel
Sep 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ansgar S OlsenRobert V Intine
Dec 3, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yung-Che TsengPung-Pung Hwang
Apr 1, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Pung-Pung HwangLi-Yih Lin
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michael P SarrasRobert V Intine
Jul 3, 2013·American Journal of Nephrology·Charles W HeiligN Stanley Nahman
Nov 29, 2013·Journal of Diabetes Research·Angela Ka Wai Lai, Amy C Y Lo
Apr 8, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Katiucia Marques CapiottiRosane Souza Da Silva
Aug 27, 2014·Behavioural Brain Research·Katiucia Marques CapiottiRosane Souza Da Silva

❮ Previous
Next ❯

Citations

Jun 25, 2016·The Journal of Comparative Neurology·Anne-Claire DorsemansNicolas Diotel
Sep 20, 2017·International Journal of Molecular Sciences·Karl Heckler, Jens Kroll
Aug 31, 2018·Disease Models & Mechanisms·Zaid TanvirVictoria P Connaughton
Sep 5, 2018·Frontiers in Cell and Developmental Biology·Liqing ZangWenbiao Chen
Feb 1, 2020·Animal Frontiers : the Review Magazine of Animal Agriculture·Tsegay TeameZhigang Zhou
May 24, 2019·Experimental Animals·Khaled BenchoulaHesham R El-Seedi
Sep 11, 2019·Endocrinology·Anne M HoubrechtsVeerle M Darras
May 1, 2021·Journal of the Electrochemical Society·Whirang ChoAlexander G Zestos

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

SPSS
Excel
SigmaPlot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.