Abstract
We previously reported the efficacy of anti-cancer therapy with hyperthermia using an alternating magnetic field (AMF) and a magnetic compound. In the course of the study, unexpectedly, we found that an AMF enhances the cytotoxicity of Compound C, an activated protein kinase (AMPK) inhibitor, although this compound is not magnetic. Therefore, we examined the cellular mechanism of AMF-induced cytotoxicity of Compound C in cultured human glioblastoma (GB) cells. An AMF (280 kHz, 250 Arms) for 30 minutes significantly enhanced the cytotoxicity of Compound C and promoted apoptosis towards several human GB cell lines in vitro. The AMF also increased Compound C-induced cell-cycle arrest of GB cells at the G2 phase and, thus, inhibited cell proliferation. The AMF increased Compound C-induced reactive oxygen species production. Furthermore, the AMF decreased ERK phosphorylation in the presence of Compound C and suppressed the protective autophagy induced by this compound. The application of an AMF in cancer chemotherapy may be a simple and promising method, which might reduce the doses of drugs used in future cancer treatment and, therefore, the associated side effects.
References
Oct 24, 2002·Bioelectromagnetics·M J Ruiz-GómezM Martínez-Morillo
Jan 7, 2004·Cancer Cell·Aimee L Edinger, Craig B Thompson
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Reuben J ShawLewis C Cantley
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Mar 31, 2005·Molecular and Cellular Biology·Claire K InmanPaul Shore
Aug 2, 2005·Cell Metabolism·Barbara B KahnD Grahame Hardie
Mar 8, 2006·Annals of Internal Medicine·Julio SoteloMiguel Angel López-González
Jun 15, 2006·Journal of Neuro-oncology·Klaus Maier-HauffAndreas Jordan
Jul 18, 2006·Cancer Cell·Kurt DegenhardtEileen White
Mar 14, 2007·Anti-cancer Drugs·Philipp BaumannRalf Schmidmaier
Apr 18, 2009·Molecular Cancer Therapeutics·Hyeon Ung ParkSean P Collins
May 12, 2009·Biochemical Pharmacology·Ljubica VucicevicVladimir Trajkovic
Jun 17, 2009·Journal of Lipid Research·Junfei JinYi-Te Hsu
Sep 17, 2010·Journal of Neuro-oncology·Klaus Maier-HauffAndreas Jordan
Oct 29, 2010·Autophagy·Ljubica VucicevicVladimir Trajkovic
Oct 19, 2012·International Journal of Oncology·Hong-Quan DuongInsoo Bae
Jan 1, 2013·Toxicology and Applied Pharmacology·Shi-Wei HuangJeng-Jer Shieh
Feb 2, 2013·Cancer Research·Marcos RíosRosa Señarís
Oct 12, 2013·Cell Death & Disease·X SuiH Pan
Jan 15, 2014·Molecular Cancer Therapeutics·Xiaona LiuBiplab Dasgupta
Mar 4, 2014·PloS One·Masanari UmemuraKousaku Iwatsubo
Mar 13, 2014·The Journal of Physiological Sciences : JPS·Itaru SatoYoshihiro Ishikawa
Aug 5, 2014·Cancer Science·Yong ZhouMantian Mi
Mar 18, 2015·Scientific Reports·Haruki EguchiYoshihiro Ishikawa
Apr 23, 2016·Scientific Reports·Itaru SatoYoshihiro Ishikawa
Feb 22, 2017·Scientific Reports·Makoto OhtakeYoshihiro Ishikawa
Mar 17, 2017·Journal of Neurosurgery·Sandeep MittalCsaba Juhász
Aug 7, 2017·Journal of Pharmacological Sciences·Masanari UmemuraYoshihiro Ishikawa
Nov 9, 2017·Neuro-oncology·Quinn T OstromJill S Barnholtz-Sloan
Apr 13, 2018·Oncotarget·Rina NakakajiYoshihiro Ishikawa
Apr 21, 2018·Scientific Reports·Masatoshi NarikawaYoshihiro Ishikawa
May 4, 2018·Physiological Reports·Ryo TanakaYoshihiro Ishikawa