Alternative ESC and ESC-like subunits of a polycomb group histone methyltransferase complex are differentially deployed during Drosophila development

Molecular and Cellular Biology
Liangjun WangJ Simon

Abstract

The Extra sex combs (ESC) protein is a Polycomb group (PcG) repressor that is a key noncatalytic subunit in the ESC-Enhancer of zeste [E(Z)] histone methyltransferase complex. Survival of esc homozygotes to adulthood based solely on maternal product and peak ESC expression during embryonic stages indicate that ESC is most critical during early development. In contrast, two other PcG repressors in the same complex, E(Z) and Suppressor of zeste-12 [SU(Z)12], are required throughout development for viability and Hox gene repression. Here we describe a novel fly PcG repressor, called ESC-Like (ESCL), whose biochemical, molecular, and genetic properties can explain the long-standing paradox of ESC dispensability during postembryonic times. Developmental Western blots show that ESCL, which is 60% identical to ESC, is expressed with peak abundance during postembryonic stages. Recombinant complexes containing ESCL in place of ESC can methylate histone H3 with activity levels, and lysine specificity for K27, similar to that of the ESC-containing complex. Coimmunoprecipitations show that ESCL associates with E(Z) in postembryonic cells and chromatin immunoprecipitations show that ESCL tracks closely with E(Z) on Ubx regulatory DNA in win...Continue Reading

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Citations

Dec 19, 2006·Nature Reviews. Genetics·Yuri B Schwartz, Vincenzo Pirrotta
Sep 10, 2009·Nature Reviews. Molecular Cell Biology·Jeffrey A Simon, Robert E Kingston
Nov 5, 2008·Molecular and Cellular Biology·Stanley M LoNicole J Francis
Aug 25, 2007·Genetics·Andrés Gaytán de Ayala AlonsoJürg Müller
Apr 7, 2009·Current Opinion in Genetics & Development·Jürg Müller, Peter Verrijzer
Mar 26, 2013·Developmental Biology·Heather Mason-SuaresPeter J Harte
Sep 25, 2012·Current Opinion in Plant Biology·Marian Bemer, Ueli Grossniklaus
Feb 16, 2008·Mechanisms of Development·Katsuhito OhnoVincenzo Pirrotta
Feb 15, 2018·Clinical Epigenetics·Sheraz KhanWasim Abbas
Aug 6, 2017·Genetics·Judith A KassisJohn W Tamkun
Dec 16, 2017·Genetics·Liangjun WangJeffrey A Simon
Jan 5, 2017·Genetics·Yuri B Schwartz, Giacomo Cavalli

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