PMID: 6403939Jan 1, 1983Paper

Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids

Proceedings of the National Academy of Sciences of the United States of America
J L SuitS E Luria

Abstract

We have studied the physiological effects of mitomycin C induction on cells carrying ColE1 plasmids with differing configurations of three genes: the structural gene coding for colicin (cea), a gene responsible for mitomycin C lethality (kil) that we located as part of an operon with cea, and the immunity (imm) gene, which lies near cea but is not in the same operon. kil is close to or overlaps imm. When cea(+) plasmids are present mitomycin C induction results in 100-fold or greater increases in the level of colicin. Within an hour after induction more than 90% of cells carrying cea(+)kil(+) plasmids are killed and macromolecular synthesis stops, capacity for transport of proline, thiomethyl beta-D-galactoside, and alpha-methyl glucoside is lost, and the membrane becomes abnormally permeable as indicated by an increased accessibility of intracellular beta-galactosidase to the substrate o-nitrophenyl beta-D-galactoside. All of these events occur when a cea(-)kil(+)imm(+) plasmid is present and none does when the plasmid is cea(+)kil(-)imm(+), so the damage can be attributed solely to the Kil function and not to the presence of colicin. However, cells carrying a cea(+)kil(-)imm(-) plasmid are killed upon induction, apparently by...Continue Reading

References

Aug 13, 1976·Biochimica Et Biophysica Acta·A M Jetten
Oct 2, 1979·Molecular & General Genetics : MGG·A ShaffermanS Cohen
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YamadaA Nakazawa

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Citations

Jan 1, 1986·Molecular & General Genetics : MGG·M Yamada, A Nakazawa
Mar 1, 1986·Molecular & General Genetics : MGG·V GeliC Lazdunski
Dec 1, 1995·FEMS Microbiology Reviews·F J van der WalB Oudega
Jul 29, 2010·The ISME Journal·Hadeel MajeedMargaret A Riley
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N S Waleh, P H Johnson
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V GeliC Lazdunski
Apr 16, 1984·European Journal of Biochemistry·M Yamada, A Nakazawa
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Aug 14, 2009·BMC Microbiology·Osnat GillorMargaret A Riley
Mar 20, 1985·Journal of Molecular Biology·H Masaki, T Ohta
Feb 5, 1991·Journal of Molecular Biology·M FrenetteC Lazdunski
Jan 1, 1984·Molecular & General Genetics : MGG·T OlschlägerV Braun
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·K M Takayama, M Inouye
Jul 15, 1992·European Journal of Biochemistry·R WallisC Kleanthous
Jun 1, 1990·Molecular & General Genetics : MGG·R E Harkness, V Braun
Aug 1, 1992·Journal of Bacteriology·J M Eraso, G M Weinstock
Nov 1, 1986·Journal of Bacteriology·M AltieriS E Luria
Nov 1, 1987·Journal of Bacteriology·B SallesG M Weinstock
Jul 1, 1985·Journal of Bacteriology·R SpanglerG Zubay
Mar 1, 1985·Journal of Bacteriology·J L SuitS E Luria
Apr 1, 1986·Journal of Bacteriology·J FreyM Belet

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