Alternative mRNA splicing generates the two ribulosebisphosphate carboxylase/oxygenase activase polypeptides in spinach and Arabidopsis

The Plant Cell
J M WernekeW L Ogren

Abstract

Sequence analysis of ribulosebisphosphate carboxylase/oxygenase (rubisco) activase cDNA and genomic clones isolated from spinach and Arabidopsis thaliana indicates that the two polypeptides of rubisco activase arise from alternative splicing of a common pre-mRNA. In spinach, two 5' splice sites are used in processing a single 137-nucleotide intron near the 3' end of the primary transcript. This intron was either removed completely or, alternatively, the first 22 nucleotides of the intervening sequence were retained in the mature rubisco activase mRNA. The 22-nucleotide auxiliary exon contains an in-frame ochre termination codon and leads to the synthesis of a 41-kilodalton polypeptide. Removal of the entire 137-nucleotide intervening sequence results in the synthesis of a larger 45-kilodalton polypeptide. Thus, alternative splicing of the spinach rubisco activase mRNA results in the synthesis of two polypeptides that are identical except for 37 additional amino acids at the C terminus of the 45-kilodalton polypeptide. This conclusion was confirmed by Cleveland peptide mapping and by N-terminal and C-terminal amino acid sequence analyses of both purified polypeptides. This method of producing the two rubisco activase polypeptide...Continue Reading

References

Apr 11, 1989·Nucleic Acids Research·J M Werneke, W L Ogren
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G GiulianoA R Cashmore
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M WernekeW L Ogren
May 17, 1985·Science·W Gilbert
Apr 6, 1971·Biochimica Et Biophysica Acta·H W Heldt, F Sauer

❮ Previous
Next ❯

Citations

Nov 1, 1990·Plant Molecular Biology·K M Schmid, J B Ohlrogge
Jan 1, 1991·Photosynthesis Research·C A RainesT A Dyer
Sep 1, 1992·Plant Molecular Biology·P VauxJ W Brown
Apr 14, 2007·Current Genetics·Stéphane D LemaireEmmanuelle Issakidis-Bourguet
Sep 11, 2012·Protoplasma·Raquel F CarvalhoPaula Duque
Sep 26, 2013·Molecular Biology Reports·Yusong JiangYizheng Zhang
Aug 1, 2007·Photosynthesis Research·Archie R Portis, Martin A J Parry
Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert L Houtz, Archie R Portis
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C A Glackin, J W Grula
Aug 13, 2009·Journal of Experimental Botany·Zoran RisticNovica Mladenov
Jan 25, 1991·Nucleic Acids Research·R WaughJ W Brown
Jun 15, 1996·Nucleic Acids Research·A S BournayG C Machray
Apr 24, 2002·The Plant Cell·Nancy A Eckardt
Dec 1, 1996·The Plant Cell·A J McCulloughM A Schuler
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·J. W. S. Brown, C. G. Simpson
Sep 12, 2002·Annual Review of Plant Biology·Robert J Spreitzer, Michael E Salvucci
Jan 16, 2007·Annual Review of Plant Biology·Anireddy S N Reddy
Jun 8, 2010·BMC Plant Biology·Adam C EnglishAnn E Loraine
Jan 12, 2002·Biochemical and Biophysical Research Communications·Arun Sharma, Setsuko Komatsu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.