PMID: 9193067May 1, 1997Paper

Alternative processing of the maize Ac transcript in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
D J MartinJ Draper

Abstract

The successful application of the maize transposable element system Ac/Ds as a genome mutagen in heterologous plant species has recently proved the versatility and power of this technique in plant molecular biology. However, the frequency of Ac/Ds transposition is considerably lower in Arabidopsis thaliana than in most other dicot plant species that have been studied. Since previous research has established that transcripts derived from monocot genes can be alternatively processed in dicot plants, we have investigated both the efficiency of intron splicing and polyadenylation of the maize Ac transposase pre-mRNA in Arabidopsis thaliana, Nicotiana tabacum, Nicotiana plumbaginifolia and Zea mays. In this paper, we demonstrate that intron 4 is alternatively spliced within Arabidopsis, using cryptic 5' and 3'splice sites within the intron sequence, leading to a heterogeneous population of full length of transposase transcript. Furthermore, analysis of transposase transcript polyadenylation revealed that at least four alternative poly(A) sites were utilized between introns 2 and 3, resulting in truncated transposase transcripts. Finally, by Northern blotting, we established that the truncated transposase transcript was the most abun...Continue Reading

Citations

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·J. W. S. Brown, C. G. Simpson
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·Stephen RutherfordIan Moore
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Ian MooreSmita Kurup
Feb 23, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Smriti GuptaShailesh K Lal

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