PMID: 7287304Mar 1, 1981Paper

Alternative reducing agents for reductive methylation of amino groups in proteins

International Journal of Peptide and Protein Research
K F GeogheganR E Feeney

Abstract

Dimethylamine borane and trimethylamine borane were compared with sodium cyanoborohydride as reducing agents for the reductive methylation of amino groups in proteins. Dimethylamine borane performed nearly as well as cyanoborohydride; either reducing agent (15 mM) with 20 mM formaldehyde gave extensive methylation of turkey ovomucoid (5 mg/ml) in 2 h at 22 degrees. Trimethylamine borane gave equivalent levels of methylation only with a higher concentration of formaldehyde, suggesting that it is an even milder reducing agent than the other two under these conditions. With all three reducing agents, the pH optimum for methylation covered a range of pH 6-9. Methylations should be performed at the lowest possible pH to avoid side reactions of formaldehyde with the protein. Possible effects of these reducing agents on the disulfide bonds of proteins were studied. No reduction of disulfides of turkey ovomucoid was observed using each of the three reducing agents under conditions for methylation.

References

Nov 27, 1979·Biochemistry·K F GeogheganR E Feeney
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Citations

Jan 31, 1997·The Journal of Biological Chemistry·Y HamaS C Li
Apr 23, 2008·Current Protocols in Protein Science·K F Geoghegan
Dec 5, 2019·Environmental Science and Pollution Research International·Anand Giri, Deepak Pant
Apr 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W R PaukovitsA M Rogan
Jan 16, 1999·The Journal of Biological Chemistry·M P DoyleB A Kerwin
Jun 28, 1995·Journal of Immunological Methods·T D BrumeanuC A Bona
Jul 29, 1983·Biochimica Et Biophysica Acta·L M GerenF Millett
May 15, 1984·Analytical Biochemistry·W S WongR E Feeney
Apr 5, 1988·Biotechnology and Bioengineering·H Kitano, N Ise
Aug 1, 1982·Analytical Biochemistry·J C CabacunganR E Feeney
Jan 1, 1987·Analytical Biochemistry·J R Panuska, C W Parker
Sep 21, 1993·Biochemistry·H D White, I Rayment
Jul 10, 2007·BMC Structural Biology·Neil ShawZhi-Jie Liu

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