PMID: 8611157Feb 1, 1996Paper

Alternative RNA splicing of the human endothelin-A receptor generates multiple transcripts

The Biochemical Journal
Y MiyamotoK Nakao

Abstract

In order to elucidate the regulatory mechanisms of expression of the human endothelin-A receptor (hET-AR) gene, we characterized hET-AR transcripts using reverse transcriptase (RT)-PCR analysis in a variety of human tissues. RT-PCR of lung mRNA using a set of primers from exons 2 and 5 showed two lower-molecular-mass transcripts in addition to the expected fragment. When RT-PCR with primers from exons 4 and 8 was performed, no transcripts other than the expected one were detected. PCR cloning utilizing a set of primers from exons 2 and 8 which covered the entire coding sequence revealed that the cDNA clones corresponding to the two novel transcripts contained deletions of 199 bp and 327 bp respectively compared with the previously described hET-AR cDNA. Comparison of their sequences with that of the hET-AR gene showed that the deleted sequences correspond exactly to exon 4 and exons 3 and 4 respectively, indicating that these lower-molecular-mass ET-AR transcripts results from alternative RNA splicing (designated ET-AR delta 4 and ET-AR delta 3,4 respectively). Alternative splicing of exon 4 results in a transcript which would be translated into a C-terminal truncated protein containing the first, second and third transmembrane...Continue Reading

Citations

Dec 12, 2001·British Journal of Pharmacology·T M Curtis, C N Scholfield
Sep 2, 2011·The Journal of Clinical Endocrinology and Metabolism·M Cervar-ZivkovicG Desoye
Feb 22, 2007·Molecular Endocrinology·Noriyuki HataeStanko S Stojilkovic
Jan 24, 2009·Annals of the New York Academy of Sciences·Stanko S StojilkovicMelanija Tomić
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Mar 10, 2016·Pharmacological Reviews·Anthony P DavenportJanet J Maguire
Aug 8, 2009·Expert Opinion on Therapeutic Targets·John-David Aubert, Lucienne Juillerat-Jeanneret
Jun 9, 2016·Journal of Medicinal Chemistry·John-David Aubert, Lucienne Juillerat-Jeanneret
Dec 30, 2008·The Journal of Pain : Official Journal of the American Pain Society·Alla KhodorovaGary Strichartz
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