Alternative RNA splicing produces multiple forms of c-Myb with unique transcriptional activities

Molecular and Cellular Biology
John P O'Rourke, Scott A Ness

Abstract

The c-Myb transcription factor regulates the proliferation and differentiation of hematopoietic cells, and activated alleles of c-myb induce leukemias and lymphomas in animals. Relatively minor changes in the structure of c-Myb protein change the genes that it regulates and can unleash its latent transforming activities. Here, quantitative assays were used to analyze the alternative splicing of human c-myb transcripts. We identified an array of variant transcripts, expressed in highly regulated, lineage-specific patterns, that were formed through the use of alternate exons 8A, 9A, 9B, 10A, 13A, and 14A. Expression levels of the different splice variant transcripts were regulated independently of one another during human hematopoietic cell differentiation, and the alternative splicing of c-myb mRNAs was increased in primary leukemia samples. The alternatively spliced c-myb transcripts were associated with polysomes and encoded a series of c-Myb proteins with identical DNA binding domains but unique C-terminal domains. In several types of assays, the variant c-Myb proteins exhibited quantitative and qualitative differences in transcriptional activities and specificities. The results suggest that the human c-myb gene encodes a fam...Continue Reading

References

Aug 1, 1991·The Journal of General Virology·J A ClaessensP J Sondermeijer
Jun 1, 1990·Molecular and Cellular Biology·G L Shen-OngJ F Mushinski
Dec 9, 1996·Biochimica Et Biophysica Acta·S A Ness
Sep 25, 1999·The Journal of Biological Chemistry·Y TanakaS Ishii
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 16, 2003·Blood Cells, Molecules & Diseases·Scott A Ness
Apr 24, 2004·The Journal of Biological Chemistry·Wanli LeiScott A Ness
Jun 15, 2004·Nature Immunology·Timothy P BenderKlaus Rajewsky
Nov 3, 2004·Cancer Research·Julian P Venables
Jun 22, 2006·Journal of Cell Science·Anabella Srebrow, Alberto R Kornblihtt
Feb 21, 2007·Nature Structural & Molecular Biology·Rotem KarniAdrian R Krainer
Apr 17, 2007·Nature Genetics·Idoya LahortigaJan Cools

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Citations

Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 25, 2008·Nature Reviews. Cancer·Robert G Ramsay, Thomas J Gonda
Jan 23, 2010·The Journal of Clinical Investigation·Shenghao JinAlan M Gewirtz
May 22, 2009·Blood·Justin WrayRobert Hromas
Jan 26, 2011·BMC Cancer·Anita M QuintanaScott A Ness
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Ye Zhou, Scott A Ness
Aug 6, 2013·Blood Cells, Molecules & Diseases·Beverly W BaronJoseph M Baron
May 27, 2014·Oral Oncology·Göran StenmanMattias K Andersson
Aug 23, 2012·Gene·Olga KelemenStefan Stamm
Mar 3, 2010·Genesis : the Journal of Genetics and Development·Stacey J BakerE Premkumar Reddy
Feb 29, 2016·Journal of Pathology and Translational Medicine·Eun Kyung KimSoo Jeong Kim
May 8, 2015·Journal of Molecular Cell Biology·Marco SilipoAlison Tyson-Capper
Jun 16, 2009·Cell Transplantation·Ru-Huei FuShinn-Zong Lin
Jan 3, 2019·Cancer Investigation·Elizabeth A Fry, Kazushi Inoue
Aug 18, 2016·Oncotarget·Piotr T WysockiMariana Brait
Sep 28, 2017·Journal of the National Cancer Institute·Scott A Ness
Jan 1, 2010·Acta Biochimica Et Biophysica Sinica·Li ChenWei Jin
Nov 4, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kazumi NakanoToshiki Watanabe

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