Alternative routes of aromatic catabolism in Pseudomonas acidovorans and Pseudomonas putida: gallic acid as a substrate and inhibitor of dioxygenases.

Journal of Bacteriology
V L Sparnins, S Dagley

Abstract

When 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) was added to Pseudomonase acidovorans growing at the expense of succinate, enzymes required for degrading homoprotocatechuate to pyruvate and succinate semialdehyde were strongly induced. These enzymes were effectively absent from cell extracts of the organism grown with 4-hydroxyphenylacetic acid, and this substrate was metabolized by the catabolic enzymes of the homogentisate pathway. Two separate ring-fission dioxygenases for 3,4,5-trihydroxybenzoic acid (gallic acid) were present in cell extracts of Pseudomonas putida when grown with syringic acid, and gallate was degraded by reactions associated with meta fission. One of the two gallate dioxygenases also attacked 3-O-methylgallic acid; the other, which did not, was induced when cells were exposed to gallate. This organism possessed ortho fission enzymes, including protocatechuate 3,4-dioxygenase (EC 1.13.11.3) and cis,cis-carboxymuconate-lactonizing enzyme (EC 5.5.1.2), after induction with 3,4-dihydroxybenzoic acid (protocatechuic acid). Gallate was a substrate for protocatechuate 3,4-dioxygenase, with a Vmax about 3% of that of protocatechuate and with an apparent Km slightly lower. Gallate was a powerful comp...Continue Reading

Citations

Feb 1, 1978·Die Naturwissenschaften·S Dagley
Apr 9, 2011·World Journal of Microbiology & Biotechnology·Danuta WojcieszyńskaKatarzyna Hupert-Kocurek
Jan 24, 2013·Biotechnology Progress·María Teresa Torres-ManceraGerardo Saucedo-Castañeda
Jan 1, 1989·Critical Reviews in Biotechnology·F S Sariaslani
Nov 18, 2004·Journal of Applied Microbiology·S P ChowdhuryA K Tripathi
Dec 4, 2020·Journal of Applied Microbiology·Yuanyuan WangXiangfeng He
Jun 1, 1980·Journal of Bacteriology·M I Donnelly, S Dagley
Jul 1, 1976·Journal of Bacteriology·V L Sparnins, P J Chapman
Dec 1, 1982·Journal of Bacteriology·P J KerstenJ D Lipscomb
Apr 1, 1978·Applied and Environmental Microbiology·Y L LeeS Dagley
Oct 1, 1978·Journal of Bacteriology·V L SparninsS Dagley
Dec 1, 1988·Applied and Environmental Microbiology·J TriasJ G Lorén
May 1, 1979·Journal of Bacteriology·V L SparninsS Dagley

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