Alternative sources of pluripotent stem cells: altered nuclear transfer

Cell Proliferation
M L Condic

Abstract

Altered nuclear transfer (ANT) is one of several methods that have been suggested for obtaining pluripotent stem cells without destroying human embryos. ANT proposes to alter the nucleus of a somatic cell and/or the cytoplasm of an enucleated oocyte such that when the two are combined, they do not produce a zygote, but rather they form a cell capable of producing pluripotent stem cells without being an embryo. The ANT proposal raises the serious question of whether it is possible to know with confidence that this procedure generates a non-embryo, rather than merely an embryo with a deficiency. Here I address the question of how embryos can be distinguished from non-embryos using scientific criteria and apply these criteria to the two forms of ANT proposed thus far: ANT combined with oocyte-assisted reprogramming (ANT-OAR) or with gene deletion (ANT-GD). I propose that the first globally coordinated event in human development, the formation of trophoblast and inner cell mass (ICM) lineages via Cdx2-Oct3/4 mutual cross-repression, is the earliest act of the embryo qua embryo; it is an operation intrinsic to an embryo as such, and entities lacking the power (potentia) for such an act cannot be considered embryos. Thus, I will argu...Continue Reading

References

Sep 1, 1978·American Journal of Obstetrics and Gynecology·A E Szulman, U Surti
Mar 1, 1988·Somatic Cell and Molecular Genetics·R J AubinM C Paterson
Feb 1, 1997·The Journal of Pathology·R A FisherE S Newlands
Apr 18, 2000·The Journal of Pathology·D T WeaverF J Paradinas
Apr 10, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Jo-Ann L StantonDavid P L Green
Aug 29, 2003·Development·Toshihiko FujimoriYo-Ichi Nabeshima
Sep 27, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard L Gardner, Timothy J Davies
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kallayanee ChawengsaksophakFelix Beck
Aug 7, 2004·Developmental Cell·Janet Rossant, Patrick P L Tam
Nov 3, 2004·The Journal of Clinical Investigation·Donald W Landry, Howard A Zucker
Jan 7, 2005·Development·Karolina Piotrowska-NitscheMagdalena Zernicka-Goetz
Mar 18, 2005·Nature·Berenika PlusaMagdalena Zernicka-Goetz
Apr 7, 2005·Journal of Cardiac Failure·David A Kass
Sep 21, 2005·The National Catholic Bioethics Quarterly·Maureen L Condic, Samuel B Condic
Feb 4, 2006·Reproductive Biomedicine Online·Nick StrelchenkoYury Verlinsky
Apr 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Moshe PritskerIhor R Lemischka
May 4, 2006·BMC Genomics·Huai LiMing Zhan
Aug 25, 2006·Nature·Irina KlimanskayaRobert Lanza
Sep 23, 2006·Stem Cells·Xin ZhangMiodrag Stojkovic
Jan 12, 2007·Nature·Maria-Elena Torres-PadillaMagdalena Zernicka-Goetz
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Jun 28, 2007·Nature Neuroscience·Maureen Condic

❮ Previous
Next ❯

Citations

Apr 20, 2010·Stem Cells and Development·Maureen L Condic, Mahendra Rao
Jul 30, 2009·Genome Medicine·Mahendra Rao, Maureen L Condic
Feb 15, 2008·Stem Cells and Development·Mahendra Rao, Maureen L Condic
Jun 28, 2016·Biotechnology Letters·Wojciech BarczakWojciech Golusiński

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Related Papers

BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy
William B Hurlbut
Medico; Europa Ausg
K GOESSWALD
Scientific American
Marissa Fessenden
© 2021 Meta ULC. All rights reserved