Alternative splicing and differential subcellular localization of the rat FGF antisense gene product.

BMC Molecular Biology
Shuo Cheng ZhangP R Murphy

Abstract

GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing of the primary FGF-AS mRNA transcript predicts multiple GFG isoforms in many species including rat. In the present study we focused on elucidating the expression and subcellular distribution of alternatively spliced rat GFG isoforms. RT-PCR and immunohistochemistry revealed tissue-specific GFG mRNA isoform expression and subcellular distribution of GFG immunoreactivity in cytoplasm and nuclei of a wide range of normal rat tissues. FGF-2 and GFG immunoreactivity were co-localized in some, but not all, tissues examined. Computational analysis identified a mitochondrial targeting sequence (MTS) in the N-terminus of three previously described rGFG isoforms. Confocal laser scanning microscopy and subcellular fractionation analysis revealed that all rGFG isoforms bearing the MTS were specifically targeted to mitochondria whereas isoforms and deletion mutants lacking the MTS were localized in the cytoplasm and nucleus. Mutation an...Continue Reading

References

Nov 17, 1992·Biochemistry·M L MichaelsJ H Miller
Feb 13, 1986·Biochemical and Biophysical Research Communications·S MiuraM Mori
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Jun 5, 1996·Biochemical and Biophysical Research Communications·A W LiP R Murphy
Apr 19, 1996·Molecular and Cellular Endocrinology·A W LiP R Murphy
Oct 11, 1996·The Journal of Biological Chemistry·M J BessmanS F O'Handley
Nov 1, 1996·European Journal of Biochemistry·M G Claros, P Vincens
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·R KneeP R Murphy
Dec 24, 1997·Molecular and Cellular Endocrinology·A W LiP R Murphy
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Jun 6, 2000·Current Biology : CB·N Pfanner
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Mar 22, 2001·EMBO Reports·M CokolB Rost
May 2, 2001·Nature Reviews. Molecular Cell Biology·N Pfanner, A Geissler
Feb 16, 2002·Bioinformatics·Hideo BannaiSatoru Miyano
Dec 19, 2003·Nucleic Acids Research·Ivica LetunicPeer Bork
Dec 8, 2004·Archives of Biochemistry and Biophysics·A S MildvanL M Amzel
Jan 6, 2005·Genome Research·Liliana FloreaGranger Sutton
Feb 24, 2005·Leukemia Research·Mark Baguma-NibashekaPaul R Murphy
Apr 6, 2005·Journal of Molecular Biology·Rajesh Nair, Burkhard Rost
May 10, 2005·The Journal of Biological Chemistry·Takahisa OgawaShigeru Shigeoka
Jun 23, 2005·Cancer·Alan G CassonDuane L Guernsey
Nov 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christie BarclayAlan G Casson
Dec 27, 2005·Cellular and Molecular Life Sciences : CMLS·A G McLennan
Jun 15, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shuo Cheng ZhangPaul R Murphy
Jan 1, 2007·CSH Protocols·David W Mount

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Citations

Jun 5, 2012·Expert Opinion on Drug Discovery·Anton Yuryev
Aug 23, 2012·Gene·Olga KelemenStefan Stamm
Feb 21, 2014·Molecular Endocrinology·Lori A McEachern, Paul R Murphy
May 12, 2016·Molecular Endocrinology·Jinjiang FanVassilios Papadopoulos
Feb 6, 2020·Biochemical Genetics·Tao ZhongHongping Zhang

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Methods Mentioned

BETA
PCR
transfection
transfections
Assay

Software Mentioned

MitoProt
ANTHEPROT
BLAST
3D
iPSORT
TargetP
Clustal W
PredictNLS
LOCtree
SMART Modular Architecture Research Tool

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