Alternative splicing controls neuronal expression of v-ATPase subunit a1 and sorting to nerve terminals.

The Journal of Biological Chemistry
Sandrine Poëa-GuyonNicolas Morel

Abstract

Vacuolar proton ATPase accumulates protons inside various intracellular organelles such as synaptic vesicles; its membrane domain V0 could also be involved in membrane fusion. These different functions could require vacuolar proton ATPases possessing different V0 subunit a isoforms. In vertebrates, four genes encode isoforms a1-a4, and a1 variants are also generated by alternative splicing. We identified a novel a1 splice variant a1-IV and showed that the two a1 variants containing exon C are specifically expressed in neurons. Single neurons coexpress a2, a1-I, and a1-IV, and these subunit a isoforms are targeted to different membrane compartments. Recombinant a2 was accumulated in the trans-Golgi network, and a1-I was concentrated in axonal varicosities, whereas a1-IV was sorted to both distal dendrites and axons. Our results indicate that alternative splicing of exon N controls differential sorting of a1 variants to nerve terminals or distal dendrites, whereas exon C regulates their neuronal expression.

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Citations

May 11, 2010·Biochemistry·Masashi ToeiMichael Forgac
Jul 21, 2012·The Journal of Biological Chemistry·Gaëlle CombeauJean-Vianney Barnier
Oct 30, 2013·The Journal of Cell Biology·Sandrine Poëa-GuyonNicolas Morel
Jun 13, 2009·Cerebral Cortex·Alexandre William MoreauPhilippe Fossier
Dec 1, 2009·Cellular and Molecular Life Sciences : CMLS·Eric J R JansenGerard J M Martens
Dec 4, 2010·FEBS Letters·Christina L PierEric A Johnson
Apr 14, 2009·Toxicon : Official Journal of the International Society on Toxinology·Michael R Baldwin, Joseph T Barbieri
Nov 1, 2007·Biochemical and Biophysical Research Communications·Shoko Kawasaki-NishiTsuyoshi Nishi
Mar 8, 2011·Journal of Neurochemistry·Oussama El Far, Michael Seagar
Mar 22, 2015·Cellular and Molecular Life Sciences : CMLS·Nicolas Morel, Sandrine Poëa-Guyon
Sep 1, 2009·Biochemical and Biophysical Research Communications·Eric M SerranoL Shannon Holliday
Jun 1, 2007·The Journal of Biological Chemistry·Patricia KreisJean-Vianney Barnier
Aug 12, 2016·American Journal of Physiology. Cell Physiology·Julianna Maria SantosSouad R Sennoune
Apr 17, 2015·Journal of Leukocyte Biology·Alice Gilman-SachsKenneth Beaman
Mar 31, 2017·Biomedical Reports·Qian-Yin XiongJia-Lin Gao
Jun 15, 2011·Molecular Cancer Research : MCR·Toshihiko NishishoToshiyuki Yoneda
Feb 22, 2007·Biochemistry·Michael R Baldwin, Joseph T Barbieri

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