Alternative Splicing in Adhesion- and Motility-Related Genes in Breast Cancer

International Journal of Molecular Sciences
Rosanna AversaValerio Costa

Abstract

Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations-in human breast cell lines and breast tumor biopsies-we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression.

References

Sep 4, 2003·The Journal of Biological Chemistry·Agnes G S H van RossumEd Schuuring
May 15, 2007·Cellular and Molecular Life Sciences : CMLS·M T ElolaG A Rabinovich
Nov 21, 2007·The Journal of Biological Chemistry·Jakub M SwierczStefan Offermanns
Mar 21, 2008·Molecular & Cellular Proteomics : MCP·Kasper ThorsenTorben F Orntoft
Mar 26, 2010·Molecular Carcinogenesis·Stephen J AssinderClare Winnick
Sep 23, 2010·Molecular Cancer·Ewe Seng Ch'ng, Atsushi Kumanogoh
Nov 3, 2010·Genes & Development·Charles J David, James L Manley
Jun 15, 2011·Advanced Drug Delivery Reviews·Trenis D PalmerAndries Zijlstra
Oct 18, 2011·Cell·Scott Valastyan, Robert A Weinberg
Dec 14, 2011·Cell Research·Atsuko SakuraiJ Silvio Gutkind
Feb 22, 2012·Cancer Research·Gaofeng XiongRen Xu
Mar 2, 2012·The Journal of Clinical Investigation·Thomas WorzfeldStefan Offermanns
Aug 23, 2012·Pharmacology & Therapeutics·Sharmistha PalRamana V Davuluri
Aug 29, 2012·Cancer Science·Hideyo MiyatoJoji Kitayama
Apr 23, 2013·Scientific Reports·Jeyanthy EswaranRakesh Kumar
May 15, 2013·Biochimica Et Biophysica Acta·Luciana D'ApicePiergiuseppe De Berardinis
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Feb 28, 2014·Database : the Journal of Biological Databases and Curation·Margherita ScarpatoValerio Costa
Jul 1, 2014·Small GTPases·Amine Sadok, Chris J Marshall
Jul 30, 2014·PloS One·Daniella Arêas Mendes-da-CruzOlivier Hermine
Mar 25, 2015·Oncotarget·Valerio CostaAlfredo Ciccodicola

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Citations

Jun 28, 2016·International Journal of Molecular Sciences·Valerio CostaAlfredo Ciccodicola
Aug 2, 2017·International Journal of Molecular Sciences·Amelia CasamassimiAlfredo Ciccodicola
Jul 15, 2017·Molecular Genetics and Genomics : MGG·Tatsiana V Ramanouskaya, Vasily V Grinev

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Datasets Mentioned

BETA
GSE68228

Methods Mentioned

BETA
biopsies
RNA-Seq
GTPase
PCR
GTPases
biopsy

Software Mentioned

Ensembl
ImageJ
LAS Software
Cufflinks
R

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