PMID: 8848255Jan 5, 1996

Alternative splicing in the coding region of human aromatic L-amino acid decarboxylase mRNA

Neuroscience Letters
Y T ChangKeith Hyland

Abstract

Total RNA from human neuroblastoma cells (SK-N-SH) was reverse transcribed and amplified using primers specific for aromatic L-amino acid decarboxylase (AADC). Two polymerase chain reaction (PCR) products were observed following agarose electrophoresis. Cycle sequencing of the PCR products revealed the larger fragment (414 bp) to be identical to the published human cDNA sequence (Type I). Sequencing of the smaller band (300 bp) demonstrated a form missing exon three (Type II). Both types of the mRNA were colocalized in human brain regions (gray matter and white matter) and other human tissues (liver, kidney, adipose, heart, adrenal gland and keratinocytes). The relative concentrations varied in each tissue studied but specific neuronal or non-neuronal patterns were not apparent. The study demonstrates alternative splicing within the coding region of the human AADC mRNA and the results suggest the possibility that two proteins are derived from the AADC gene in human tissues.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V R AlbertA Aguanno
Oct 1, 1991·Neuroscience Research·T Nagatsu
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M KriegerJ Thibault
Nov 15, 1989·Biochemical and Biophysical Research Communications·H IchinoseT Nagatsu
Jan 1, 1988·Neuroscience and Biobehavioral Reviews·K DakshinamurtiY L Siow
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·U B Gyllensten, H A Erlich
Jan 1, 1985·Experimental Brain Research·Y L Siow, K Dakshinamurti
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G ChristensonS Udenfriend
Mar 1, 1993·Brain Research. Molecular Brain Research·A Le Van ThaiM J Weber

Citations

May 5, 2011·Journal of Inherited Metabolic Disease·Riccardo MontioliCarla Borri Voltattorni
Jun 11, 2010·Molecular Biology Reports·Ioanna ChalatsaDido Vassilacopoulou
Jun 9, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Vachtenheim, H Novotná
Jul 1, 2008·Clinical Biochemistry·Margaritis AvgerisAndreas Scorilas
Jun 24, 2008·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Hsiu-Fen LeeHuei-Jane Lee
Jun 28, 2008·Neurochemistry International·Regina C C KubruslyFernando G de Mello
Aug 5, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D KeszthelyiA A M Masclee
Mar 3, 2004·Annals of Neurology·Yuh Terng ChangKeith Hyland
Jun 27, 2018·Pharmacological Reviews·Raul R GainetdinovMark D Berry
May 11, 2019·The Journal of Clinical Endocrinology and Metabolism·Julie Le MestreEstelle Louiset

Related Concepts

Aromatic-L-Amino-Acid Decarboxylases
Brain Tumor, Recurrent
Electrophoresis, Agar Gel
Neuroblastoma
RNA, Messenger, Splicing
Poly(A) Tail
Nested Polymerase Chain Reaction

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