PMID: 8600184Dec 1, 1995Paper

Alternative splicing of amelogenin mRNA from rat incisor ameloblasts

Journal of Dental Research
R S LiP K Denbesten

Abstract

Amelogenin proteins are a major component of the developing enamel matrix, and are likely to have a key role in the control of enamel biomineralization. The heterogeneity of amelogenin is in part due to alternative splicing of the amelogenin RNA transcripts. Several patterns of alternative splicing have been described in mouse, bovine, porcine, and human enamel, with all alternatively spliced products having homologous 5' and 3' sequences within the coding regions. In these studies, we have used anchored PCR to identify alternatively spliced amelogenin cDNA sequences in the rat. We found amelogenin cDNAs that could be divided into two groups based on their 3' sequence. Group 1 cDNAs had a novel terminal sequence that has not been previously identified, while group 2 cDNAs were similar to those previously identified in other animal species. We identified a sequence, identical to the novel 3' amelogenin cDNA sequence, in rat genomic DNA downstream from the previously identified exon 7. The putative amelogenin proteins predicted for the two groups differed in their predicted isoelectric points, with the novel group 1 proteins having a more basic isoelectric point.

References

Mar 1, 1979·Journal of Dental Research·M ShimizuM Fukae
Nov 26, 1992·Biochemical and Biophysical Research Communications·E C LauA G Fincham
Jan 1, 1991·Archives of Oral Biology·S SasakiM Suzuki
Jan 29, 1991·Biochemistry·C GibsonJ Rosenbloom
Feb 14, 1991·Biochemical and Biophysical Research Communications·C W GibsonJ Rosenbloom
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jan 1, 1989·Archives of Oral Biology·J CarterM G Shepherd
Dec 1, 1987·Advances in Dental Research·M F YoungJ D Termine
Jun 28, 1985·Biochemical and Biophysical Research Communications·M L SneadH C Slavkin
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Oct 1, 1994·Calcified Tissue International·J P SimmerA G Fincham
Jan 1, 1994·Calcified Tissue International·Y YamakoshiM Shimizu
Jul 30, 1960·Nature·J E EASTOE

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Citations

Feb 8, 2005·Journal of Molecular Evolution·Sidney DelgadoJean-Yves Sire
Jun 1, 2012·Journal of Molecular Histology·Junsheng FengShuo Chen
Apr 14, 2006·Cells, Tissues, Organs·Carolyn W GibsonJ Timothy Wright
Sep 26, 2006·Journal of Dental Research·J D BartlettJ P Simmer
Apr 25, 2008·Journal of Dental Research·K TanimotoW Li
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·S ToyosawaJ Klein
Dec 20, 2002·Connective Tissue Research·James P Simmer, Jan C C Hu
Nov 4, 2000·Connective Tissue Research·P K Den BestenW Li
Aug 26, 1998·Journal of Comparative Pathology·Y YuasaB R Madewell
Jun 17, 2005·Archives of Oral Biology·Yong LiCarolyn W Gibson
Aug 26, 1998·Archives of Oral Biology·W LiP K DenBesten
Jun 12, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Jonathan StahlPamela DenBesten
Aug 12, 1999·Journal of Structural Biology·A G FinchamJ P Simmer
Jun 24, 2005·Journal of Dental Research·P PapagerakisM MacDougall
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C E Smith
Aug 20, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Otto BabaYoshiro Takano
Mar 21, 2015·Journal of Dental Research·J StahlP K Den Besten
Sep 29, 2011·Journal of Dental Research·J-Y SireP K Denbesten
Mar 13, 2009·Journal of Dental Research·Y YagiK Moriyama
May 6, 2006·European Journal of Oral Sciences·T G H DiekwischX Luan
Mar 4, 2005·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
Mar 1, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
Jan 5, 2000·Journal of Cellular Biochemistry·P PapagerakisA Berdal
May 7, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yulei HuangYan Zhang

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