Alternative splicing of Drosophila Dscam generates axon guidance receptors that exhibit isoform-specific homophilic binding

Cell
Woj M WojtowiczJames C Clemens

Abstract

Dscam is an immunoglobulin (Ig) superfamily protein required for the formation of neuronal connections in Drosophila. Through alternative splicing, Dscam potentially gives rise to 19,008 different extracellular domains linked to one of two alternative transmembrane segments, resulting in 38,016 isoforms. All isoforms share the same domain structure but contain variable amino acid sequences within three Ig domains in the extracellular region. We demonstrate that different isoforms exhibit different binding specificity. Each isoform binds to itself but does not bind or binds poorly to other isoforms. The amino acid sequences of all three variable Ig domains determine binding specificity. Even closely related isoforms sharing nearly identical amino acid sequences exhibit isoform-specific binding. We propose that this preferential homophilic binding specificity regulates interactions between cells and contributes to the formation of complex patterns of neuronal connections.

References

Jul 19, 1996·The Journal of Biological Chemistry·J C ClemensJ E Dixon
Nov 15, 1996·Science·M Tessier-Lavigne, C S Goodman
Feb 4, 1998·Trends in Genetics : TIG·M Missler, T C Südhof
Apr 8, 1998·Annual Review of Neuroscience·J G Flanagan, P Vanderhaeghen
Aug 5, 2000·Brain Research. Molecular Brain Research·K L AgarwalaK Yamakawa
Aug 26, 2000·Science·M HattoriJ G Flanagan
Oct 5, 2002·Science·Robert A HoltStephen L Hoffman
Feb 21, 2003·Current Opinion in Neurobiology·Todd McLaughlinDennis D M O'Leary
Oct 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R W SPERRY

❮ Previous
Next ❯

Citations

Jan 29, 2011·Neuroscience Bulletin·Kun ZhuHaihong Ye
Sep 10, 2011·Protein & Cell·Yiliang XuJane Y Wu
May 15, 2013·Fish & Shellfish Immunology·Jingjing WangLinsheng Song
Jul 21, 2010·Expert Reviews in Molecular Medicine·Dirk BäumerKevin Talbot
Sep 19, 2012·Cell Research·Phuong Hoang, Wesley B Grueber
Jun 8, 2007·Nature·S Sean MillardS Lawrence Zipursky
Sep 14, 2007·Nature·Daisuke HattoriBarry J Dickson
Jan 25, 2008·Nature·Peter G FuerstRobert W Burgess
Sep 20, 2007·Nature Immunology·Thomas Boehm
Feb 19, 2013·Nature Neuroscience·Allissa A DillmanMark R Cookson
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Arianne J MatlinChristopher W J Smith
Apr 21, 2010·Nature Reviews. Neuroscience·Yuh-Nung Jan, Lily Yeh Jan
Dec 5, 2006·Nucleic Acids Research·Michael HillerMatthias Platzer
Jan 3, 2012·Nucleic Acids Research·Michal Golan-MashiachEhud Shapiro
Jun 25, 2010·Cold Spring Harbor Perspectives in Biology·Wesley B Grueber, Alvaro Sagasti
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·G Neves, A Chess
Aug 18, 2006·Molecular and Cellular Biology·Shinichi YonekuraChi-Hon Lee
Jan 5, 2012·Molecular and Cellular Biology·Grace J Lah, Brian Key
Jul 12, 2013·Annual Review of Neuroscience·S Lawrence Zipursky, Wesley B Grueber
Oct 8, 2008·Annual Review of Cell and Developmental Biology·Daisuke HattoriS Lawrence Zipursky
Apr 7, 2010·Annual Review of Neuroscience·Kang Shen, Peter Scheiffele
Jun 30, 2007·Annual Review of Neuroscience·Lawrence ShapiroDavid R Colman
Feb 18, 2006·BMC Evolutionary Biology·Mack E CraytonMorgan C Giddings
Dec 24, 2009·BMC Genomics·Krishanpal AnamikaNarayanaswamy Srinivasan
Oct 13, 2010·Molecular Brain·Scott Cameron, Yong Rao
Mar 2, 2006·Genome Biology·Dimitris AnastassiouVinay Varadan
Mar 10, 2009·Development·Megan M CortyWesley B Grueber
Jun 23, 2011·PLoS Pathogens·Apiruck WatthanasurorotKenneth Söderhäll
Feb 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hung-Hsiang YuTzumin Lee
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aljoscha NernS Lawrence Zipursky
Dec 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Joshua A WeinerJoshua R Sanes
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael L TressAlfonso Valencia
Apr 15, 2014·Developmental and Comparative Immunology·Tze Hann NgHan-Ching Wang
Aug 2, 2014·Developmental and Comparative Immunology·Tze Hann NgHan-Ching Wang
Mar 19, 2014·Nature Biotechnology·Nathan BoleyJames B Brown
Apr 12, 2008·Molecular Biology and Evolution·Daniela BritesLouis Du Pasquier
Jan 14, 2009·Journal of Neurogenetics·Yoshinori AsoHiromu Tanimoto
Jun 25, 2014·Journal of Neurogenetics·Carmen Mohr, Britta Hartmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.