Alternative splicing of fibroblast growth factor receptor 2 (FGF-R2) in human prostate cancer

Oncogene
R P CarstensM A Garcia-Blanco

Abstract

Progression of prostate cancer from an androgen sensitive to androgen insensitive tumor has previously been shown to be accompanied by a change in alternative splicing of fibroblast growth factor receptor 2 (FGF-R2) in a rat model of prostate cancer. This change results in loss of the FGF-R2(IIIb) isoform and predominant expression of the FGF-R2(IIIc) isoform. We sought to determine whether this change in FGF-R2 splicing is also associated with androgen insensitivity in human prostate tumors. We analysed three well characterized human prostate cancer cell lines and three metastatic prostate tumors which have been maintained as xenografts in nude mice. One of the cell lines, LNCaP, and two of the xenografts, DUKAP-1 and DUKAP-2, have been characterized as androgen sensitive, whereas two of the cell lines, DU-145 and PC-3, and one of the xenografts, DU9479, display androgen independent growth. Using an RT-PCR based assay, we demonstrated that progressive loss of the FGF-R2(111b) isoform correlated with androgen insensitivity in these human prostate cancer models. These findings lend support to the hypothesis that that loss of FGF-R2(IIIb) may be one step in a series of events which lead to progression of human prostate cancer.

Citations

Nov 10, 2000·International Journal of Cancer. Journal International Du Cancer·E ValveP Härkönen
Nov 25, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·A MatsubaraT Usui
Aug 1, 2013·Apoptosis : an International Journal on Programmed Cell Death·Kai Hung TiongChee-Onn Leong
Jun 20, 2008·Breast Cancer Research and Treatment·Anita L BaneIrene L Andrulis
Jul 28, 2001·Progress in Neurobiology·P J Grabowski, D L Black
Oct 31, 2002·RNA·Bianca J Lam, Klemens J Hertel
May 5, 2000·The Biochemical Journal·B D Lichty, S Kamel-Reid
Jun 22, 2006·Journal of the National Cancer Institute·Paul W Finch, Jeffrey S Rubin
Jan 18, 2006·Nucleic Acids Research·Ruben H HovhannisyanRuss P Carstens
Nov 3, 2012·Nucleic Acids Research·Anke Busch, Klemens J Hertel
Nov 3, 2010·Genes & Development·Charles J David, James L Manley
Jun 25, 2008·Molecular and Cellular Biology·David M MaugerMariano A Garcia-Blanco
Apr 25, 2000·Molecular and Cellular Biology·L YangD D Hickstein
Feb 2, 2006·Molecular and Cellular Biology·Andrew P BaraniakMariano A Garcia-Blanco
Oct 23, 2008·Journal of Bioinformatics and Computational Biology·Thomas K F WongS M Yiu
Dec 4, 2012·Journal of Cell Science·Giulio AucielloJoshua Z Rappoport
May 9, 2008·PLoS Biology·Kerstin B MeyerBruce A J Ponder
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maria SimarroPaul Anderson
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangT Okamoto
May 17, 2014·Asian Journal of Andrology·Anna V LapukColin C Collins
Feb 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qi ZhaoJun Yao
Jun 17, 2008·Cancer Letters·Mark A Reynolds
Mar 21, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Julian P Venables

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